Probe:	Wnk1 cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Semi-quantitative evaluation was summarized in Table 1, 2, and 4.

Structure	Level	Pattern	Image	Note
TS17: brain	Moderate	Ubiquitous	1B upper
TS17: future spinal cord	Moderate	Ubiquitous	1B upper
TS17: liver	Moderate	Ubiquitous	1B upper
TS17: spinal ganglion	Moderate	Ubiquitous	1B upper
TS17: heart	Moderate	Ubiquitous	1B upper	Stronger expression expression than Wnk1/Hsn2.

Structure	Level	Pattern	Image	Note
TS20: brain	Weak	Ubiquitous	1B lower
TS20: spinal cord	Weak	Ubiquitous	1B lower
TS20: spinal ganglion	Weak	Ubiquitous	1B lower
TS20: heart	Moderate	Ubiquitous	1B lower
TS20: liver	Moderate	Ubiquitous	1B lower

Structure	Level	Pattern	Image	Note
TS23: brain	Weak	Uniform	2B
TS23: heart	Weak	Uniform	2B
TS23: liver	Weak	Uniform	2B
TS23: spinal cord	Weak	Uniform	2B
TS23: spinal ganglion	Weak	Uniform	2B

Structure	Level	Pattern	Image	Note
TS27: olfactory epithelium	Strong	Non-Uniform	2F	Heterogeneousm distribution.
TS27: thymus	Strong	Non-Uniform	2F	Heterogeneousm distribution.
TS27: brain	Weak	Non-Uniform	2F
TS27: spinal ganglion	Weak	Non-Uniform	2F
TS27: liver	Trace	Non-Uniform	2F	Very weak.
TS27: spinal cord	Trace	Non-Uniform	2F	Very weak.
TS27: metanephros	Moderate	Non-Uniform	2F
TS27: trigeminal ganglion	Moderate	Non-Uniform	2F
TS27: heart	Moderate	Non-Uniform	2F	Stronger expression than Wnk1/Hsn2.
TS27: lung	Moderate	Non-Uniform	2F	Stronger expression than Wnk1/Hsn2.
TS27: intervertebral disc	Strong		2F

Structure	Level	Pattern	Image	Note
TS27: dorsal root ganglion	Present		3A
TS27: spinal cord	Present	Regionally restricted	3A	Expressed in the ventral, ventromedial and medial nuclei.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Strong		4A

Structure	Level	Pattern	Image	Note
TS28: spinal nerve	Strong	Regionally restricted	4B	Expressed in the dorsal root.

Structure	Level	Pattern	Image	Note
TS27: myenteric nerve plexus	Present		5A	Weaker expression than Wnk1/Hsn2.

Structure	Level	Pattern	Image	Note
TS28: choroid plexus	Strong		5D	Stronger expression than Wnk1/Hsn2.
TS28: habenula	Strong		5D	Stronger expression than Wnk1/Hsn2.

Structure	Level	Pattern	Image	Note
TS28: olfactory epithelium	Strong	Non-Uniform	6B	Heterogeneousm distribution.
TS28: brain	Weak	Non-Uniform	6B
TS28: liver	Weak	Non-Uniform	6B
TS28: lung	Weak	Non-Uniform	6B
TS28: metanephros	Strong	Non-Uniform	6B	More prominent expression than P1.
TS28: thymus	Strong	Non-Uniform	6B	More prominent expression than P1.
TS28: heart	Moderate	Non-Uniform	6B
TS28: spinal cord	Moderate	Non-Uniform	6B
TS28: spinal ganglion	Moderate	Non-Uniform	6B
TS28: trigeminal ganglion	Trace	Non-Uniform	6B	Very weak.
TS28: spleen	Moderate	Non-Uniform	6B	More prominent expression than P1.
TS28: lymph node	Present	Non-Uniform	6B	More prominent expression than P1.

Structure	Level	Pattern	Image	Note
TS28: olfactory epithelium	Very strong	Non-Uniform	6F	Heterogeneousm distribution.
TS28: testis	Strong	Non-Uniform	6F	Stronger expression than Wnk1/Hsn2.
TS28: brain	Weak	Non-Uniform	6F
TS28: heart	Weak	Non-Uniform	6F
TS28: spinal ganglion	Weak	Non-Uniform	6F
TS28: trigeminal ganglion	Weak	Non-Uniform	6F
TS28: metanephros	Moderate	Non-Uniform	6F
TS28: spleen	Moderate	Non-Uniform	6F
TS28: liver	Trace	Non-Uniform	6F
TS28: lung	Trace	Non-Uniform	6F
TS28: spinal cord	Trace	Non-Uniform	6F
TS28: thymus	Strong	Non-Uniform	6F

Structure	Level	Pattern	Image	Note
TS28: hair root sheath matrix	Strong		7A	Increased expression. Stronger expression than Wnk1/Hsn2.
TS28: hair outer root sheath	Moderate		7A	Increased medium expression.
TS28: hair inner root sheath	Weak		7A
TS28: hair medulla	Weak		7A

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present		8A
TS28: spinal nerve	Present	Regionally restricted	8A	A more evenly distributed expression in Schwann cells in the ventral roots.

Structure	Level	Pattern	Image	Note
TS28: trigeminal ganglion	Present		9D	Detected in the somata of the neurons and the supporting satellite cells.

Structure	Level	Pattern	Image	Note
TS28: trigeminal ganglion	Present		9G

Structure	Level	Pattern	Image	Note
TS28: hippocampus CA2	Present		10A	Increased expression.
TS28: hippocampus CA3	Present		10A	Increased expression.
TS28: hippocampus CA1	Present		10A

Structure	Level	Pattern	Image	Note
TS28: distal convoluted tubule	Strong		11A
TS28: glomerular tuft	Ambiguous		11A	Little to no labeling detected.

Structure	Level	Pattern	Image	Note
TS28: glomerular tuft	Ambiguous		11D	Little to no labeling detected.
TS28: distal convoluted tubule	Strong		11D

Structure	Level	Pattern	Image	Note
TS28: seminiferous tubule	Present		12A	Expressed in the wall. Greater expression than Wnk1/Hsn2.
TS28: spermatogonium	Present		12A	Greater expression than Wnk1/Hsn2.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present	Regionally restricted	S2D	Detected in the somata of the neurons and the supporting satellite cells.

Structure	Level	Pattern	Image	Note
TS28: dorsal root ganglion	Present	Regionally restricted	S2G	Detected in the somata of the neurons and the supporting satellite cells.