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Gene Expression Data
Assay Details
Assay
Reference: J:195918 Vieux-Rochas M, et al., BMP-mediated functional cooperation between Dlx5;Dlx6 and Msx1;Msx2 during mammalian limb development. PLoS One. 2013;8(1):e51700
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5810609
Gene symbol: Msx2
Gene name: msh homeobox 2
Reporter: lacZ
Direct Detection
Results
Specimen 1G: embryonic day 10.5; Msx2tm1Bero/Msx2+ (more )
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Present 1G
TS17: forelimb bud mesenchyme Present Regionally restricted 1G Expression in anterior and posterior mesoderm territories.

Specimen 1H: embryonic day 10.5; Msx2tm1Bero/Msx2+ (more )
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Present 1H
TS17: forelimb bud mesenchyme Present Regionally restricted 1H

Specimen 1M: embryonic day 11.5; Msx2tm1Bero/Msx2+ (more )
Structure Level Pattern Image Note
TS19: handplate apical ectodermal ridge Present 1M
TS19: forelimb bud mesenchyme Present Regionally restricted 1M Expression in larger region underneath the AER.

Specimen 1N: embryonic day 11.5; Msx2tm1Bero/Msx2+ (more )
Structure Level Pattern Image Note
TS19: handplate apical ectodermal ridge Present 1N
TS19: handplate mesenchyme Present Regionally restricted 1N Expression in larger region underneath the AER.

Specimen 1U: embryonic day 10.5; Msx2tm1Bero/Msx2+ (more )
Structure Level Pattern Image Note
TS17: hindlimb bud apical ectodermal ridge Present 1U
TS17: hindlimb bud mesenchyme Present Regionally restricted 1U Expression in anterior and posterior mesoderm territories.

Specimen 1V: embryonic day 10.5; Msx2tm1Bero/Msx2+ (more )
Structure Level Pattern Image Note
TS17: hindlimb bud apical ectodermal ridge Present 1V
TS17: hindlimb bud mesenchyme Present Regionally restricted 1V Expression in anterior and posterior mesoderm territories.

Specimen 1AA: embryonic day 11.5; Msx2tm1Bero/Msx2+ (more )
Structure Level Pattern Image Note
TS19: footplate apical ectodermal ridge Present 1AA
TS19: hindlimb bud mesenchyme Present Regionally restricted 1AA Expression in larger region underneath the AER.

Specimen 1AA': embryonic day 11.5; Msx2tm1Bero/Msx2+ (more )
Structure Level Pattern Image Note
TS19: footplate apical ectodermal ridge Strong 1AA'
TS19: footplate mesenchyme Present Regionally restricted 1AA' Expression in larger region underneath the AER.

Specimen 1AB: embryonic day 11.5; Msx2tm1Bero/Msx2+ (more )
Structure Level Pattern Image Note
TS19: footplate apical ectodermal ridge Present 1AB
TS19: footplate mesenchyme Present Regionally restricted 1AB Expression in larger region underneath the AER.

Specimen 2I: embryonic day 10.5; Msx2tm1Bero/Msx2+ (more )
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Present 2I
TS17: forelimb bud mesenchyme Present Regionally restricted 2I Expression in anterior and posterior mesoderm.

Specimen 2J: embryonic day 10.5; Msx2tm1Bero/Msx2+ (more )
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Present 2J
TS17: forelimb bud mesenchyme Present Regionally restricted 2J Expression in anterior and posterior mesoderm.

Specimen 2K: embryonic day 10.5; Dlx5/Dlx6tm1Levi/Dlx5/Dlx6tm1Levi, Dlx5/Dlx6tm1Levi/Dlx5/Dlx6tm1Levi, Msx2tm1Bero/Msx2+ (more )
Note: This embryo was a single homozygote for the Dl5/6 allele. However, since that allele affects two genes, the MGI database schema requires that we need to enter an allele pair per affected gene (thus resulting in the animal looking like a double homozygote).
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Present 2K No changes in expression compared with wildtype.
TS17: forelimb bud mesenchyme Present Regionally restricted 2K No changes in expression compared with wildtype.

Specimen 2L: embryonic day 10.5; Dlx5/Dlx6tm1Levi/Dlx5/Dlx6tm1Levi, Dlx5/Dlx6tm1Levi/Dlx5/Dlx6tm1Levi, Msx2tm1Bero/Msx2+ (more )
Note: This embryo was a single homozygote for the Dl5/6 allele. However, since that allele affects two genes, the MGI database schema requires that we need to enter an allele pair per affected gene (thus resulting in the animal looking like a double homozygote).
Structure Level Pattern Image Note
TS17: forelimb bud apical ectodermal ridge Present 2L No changes in expression compared with wildtype.
TS17: forelimb bud mesenchyme Present Regionally restricted 2L No changes in expression compared with wildtype.

Specimen 2M: embryonic day 10.5; Msx2tm1Bero/Msx2+ (more )
Structure Level Pattern Image Note
TS17: hindlimb bud apical ectodermal ridge Present 2M
TS17: hindlimb bud mesenchyme Present Regionally restricted 2M Expression in anterior and posterior mesoderm.

Specimen 2N: embryonic day 10.5; Msx2tm1Bero/Msx2+ (more )
Structure Level Pattern Image Note
TS17: hindlimb bud apical ectodermal ridge Present 2N
TS17: hindlimb bud mesenchyme Present Regionally restricted 2N Expression in anterior and posterior mesoderm.

Specimen 2O: embryonic day 10.5; Dlx5/Dlx6tm1Levi/Dlx5/Dlx6tm1Levi, Dlx5/Dlx6tm1Levi/Dlx5/Dlx6tm1Levi, Msx2tm1Bero/Msx2+ (more )
Note: This embryo was a single homozygote for the Dl5/6 allele. However, since that allele affects two genes, the MGI database schema requires that we need to enter an allele pair per affected gene (thus resulting in the animal looking like a double homozygote).
Structure Level Pattern Image Note
TS17: hindlimb bud apical ectodermal ridge Weak 2O Expression reduced in AER of hindlimb.
TS17: hindlimb bud mesenchyme Weak Regionally restricted 2O Expression reduced in anterior hindlimb mesoderm.

Specimen 2P: embryonic day 10.5; Dlx5/Dlx6tm1Levi/Dlx5/Dlx6tm1Levi, Dlx5/Dlx6tm1Levi/Dlx5/Dlx6tm1Levi, Msx2tm1Bero/Msx2+ (more )
Note: This embryo was a single homozygote for the Dl5/6 allele. However, since that allele affects two genes, the MGI database schema requires that we need to enter an allele pair per affected gene (thus resulting in the animal looking like a double homozygote).
Structure Level Pattern Image Note
TS17: hindlimb bud apical ectodermal ridge Weak 2P Expression reduced in AER of hindlimb.
TS17: hindlimb bud mesenchyme Weak Regionally restricted 2P Expression reduced in anterior hindlimb mesoderm.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/21/2024
MGI 6.23
The Jackson Laboratory