Gene Expression Data
Assay Details
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Assay
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Reference:
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J:59282
Yi SE, et al., The type I BMP receptor BMPRIB is required for chondrogenesis in the mouse limb. Development. 2000 Feb;127(3):621-30
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5806796
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Gene symbol:
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Gdf5
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Gene name:
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growth differentiation factor 5
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Probe:
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Gdf5 cDNA7
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Probe preparation:
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Antisense RNA
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Results
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Specimen
3c Wild type:
embryonic day 12.0
(more  )
Specimen
3c Wild type:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hand |
Present |
Regionally restricted |
3c wild type
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Expression was detected in regions of future joint formation.
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Specimen
3c BmprIB-/-:
(close )
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Genetic Background:
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involves: 129S/SvEv * C57BL/6J
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Mutant Allele(s):
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Bmpr1btm1Kml/Bmpr1btm1Kml
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hand |
Present |
Regionally restricted |
3c BmprIB-/-
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No difference was detected in the expression pattern in the mutant.
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Specimen
3d Wild type:
embryonic day 13.0
(more )
Specimen
3d Wild type:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
3d BmprIB-/-:
(close )
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Genetic Background:
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involves: 129S/SvEv * C57BL/6J
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Mutant Allele(s):
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Bmpr1btm1Kml/Bmpr1btm1Kml
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Age:
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embryonic day 13.0
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: forelimb digit |
Present |
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3d BmprIB-/-
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Expression in the digital ray was expanded in the mutant relative to the wild type pattern.
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Specimen
3e Wild type:
embryonic day 13.5
(more )
Specimen
3e Wild type:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
3e BmprIB-/-:
(close )
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Genetic Background:
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involves: 129S/SvEv * C57BL/6J
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Mutant Allele(s):
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Bmpr1btm1Kml/Bmpr1btm1Kml
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: forelimb digit |
Present |
Regionally restricted |
3e BmprIB-/-
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In the mutant, a single elongated domain of expression was observed in the region of the digital ray corresponding to the proximal and middle phalanges.
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| TS21: forelimb interphalangeal joint |
Weak |
Regionally restricted |
3e BmprIB-/-
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Expression in the future synovial capsule of the interphalangeal joint is greatly reduced in the mutant.
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Specimen
4c:
(close )
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Genetic Background:
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involves: 129S/SvEv * C57BL/6J
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Mutant Allele(s):
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Bmpr1btm1Kml/Bmpr1btm1Kml
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: forelimb digit |
Present |
Regionally restricted |
4c
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Expression was detected in the phalangeal region.
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Specimen
4g Wild type:
embryonic day 13.5
(more )
Note: Also labeled for BrdU.
Specimen
4g Wild type:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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Also labeled for BrdU.
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Specimen
4g BmprIB-/-:
(close )
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Genetic Background:
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involves: 129S/SvEv * C57BL/6J
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Mutant Allele(s):
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Bmpr1btm1Kml/Bmpr1btm1Kml
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Note:
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Also labeled for BrdU.
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Specimen
5a,c:
embryonic day 12.5
(more )
Note: Overlay of adjacent sections; green - Gdf; purple - Bmpr1b.
Specimen
5a,c:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Overlay of adjacent sections; green - Gdf; purple - Bmpr1b.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: limb |
Present |
Regionally restricted |
5c
,
5a
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Gdf5 and Bmpr1b are expressed in nonoverlapping, but adjacent regions of the limb.
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Specimen
5d,f:
embryonic day 13.5
(more )
Note: Overlay of adjacent sections; green - Gdf; purple - Bmpr1b.
Specimen
5d,f:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Overlay of adjacent sections; green - Gdf; purple - Bmpr1b.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: limb |
Present |
Regionally restricted |
5d
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5f
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Gdf5 and Bmpr1b are expressed in nonoverlapping, but adjacent regions of the limb.
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Analysis Tools