Gene Expression Data
Assay Details
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Assay
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Reference:
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J:14491
Dolle P, et al., Local alterations of Krox-20 and Hox gene expression in the hindbrain suggest lack of rhombomeres 4 and 5 in homozygote null Hoxa-1 (Hox-1.6) mutant embryos. Proc Natl Acad Sci U S A. 1993 Aug 15;90(16):7666-70
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5805918
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Gene symbol:
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Hoxb1
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Gene name:
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homeobox B1
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Probe:
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Hox-2.9 probe3
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Probe preparation:
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Antisense RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
2a:
embryonic day 9.5
(more  )
Specimen
2a:
(close  )
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Genetic Background:
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involves: 129S2/SvPas
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
2b:
embryonic day 9.5; Hoxa1tm1Ipc/ Hoxa1tm1Ipc
(more )
Note: (c) is a higher magnification of the boxed area in (b).
Specimen
2b:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Hoxa1tm1Ipc/Hoxa1tm1Ipc
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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(c) is a higher magnification of the boxed area in (b).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: rhombomere |
Present |
Single cells |
2b b-1
,
2c b-1
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Expression is in a small dorsal area facing the constriction between the third and fourth rhombomeres.
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Specimen
not shown E8.5-/-:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Hoxa1tm1Ipc/Hoxa1tm1Ipc
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: embryo |
Present |
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Expression in posterior regions of the mutant embryo is the same as in wild-type.
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Specimen
not shown E9.5-/-:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Hoxa1tm1Ipc/Hoxa1tm1Ipc
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Present |
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Expression in posterior regions of the mutant embryo is the same as in wild-type.
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Analysis Tools
