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Gene Expression Data
Assay Details
Assay
Reference: J:234183 Morita T, et al., An in situ hybridization study of Hyaluronan synthase (Has) mRNA in developing mouse molar and incisor tooth germs. Gene Expr Patterns. 2016 May;21(1):28-40
Assay type: RNA in situ
MGI Accession ID: MGI:5804795
Gene symbol: Has3
Gene name: hyaluronan synthase 3
Probe: Has3 cDNA2
Probe preparation: Antisense labelled with S35 RNA
Visualized with: Autoradiography
Results
Specimen 2c: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: dental placode Weak 2c
Specimen 2f: embryonic day 12.0 (more )
Structure Level Pattern Image Note
TS20: dental placode Present Regionally restricted 2f Expression is at the lingual side of the tooth bud.
Specimen 2i: embryonic day 13.0 (more )
Note: Bud stage tooth.
Structure Level Pattern Image Note
TS21: enamel organ Present 2i
Specimen 2l: embryonic day 14.0 (more )
Note: Early cap stage tooth.
Structure Level Pattern Image Note
TS22: inner enamel epithelium Very strong 2l
TS22: stellate reticulum Very strong 2l
TS22: outer enamel epithelium Absent 2l
TS22: primary enamel knot Absent 2l
Specimen 3c: embryonic day 15.0 (more )
Note: Late cap stage tooth.
Structure Level Pattern Image Note
TS23: molar enamel organ Present Regionally restricted 3c Expression is in the inner enamel epithelium and the stellate reticulum.
Specimen 3f: embryonic day 16.0 (more )
Note: Early bell stage tooth.
Structure Level Pattern Image Note
TS24: molar enamel organ Present 3f Expression is in the inner enamel epithelium, the stellate reticulum, and in the stratum intermedium.
TS24: inner enamel epithelium Present 3f
TS24: stellate reticulum Present 3f
TS24: stratum intermedium Present 3f
Specimen 3i,k: embryonic day 18.0 (more )
Note: (k) is a higher magnification of the boxed region in (i).
Structure Level Pattern Image Note
TS26: molar enamel organ Present Regionally restricted 3i , 3k Expression is in the inner enamel epithelium, the stellate reticulum, and in the stratum intermedium. Absent in differentiating ameloblasts.
TS26: inner enamel epithelium Present 3i , 3k
TS26: stellate reticulum Present 3i , 3k
TS26: stratum intermedium Present 3i , 3k
Specimen 4b: postnatal day 4 (more )
Structure Level Pattern Image Note
TS28: molar enamel organ Present Regionally restricted 4b Expression is in Hertwig's epithelial root sheath and the stratum intermedium, absent in stellate reticulum.
TS28: Hertwig's epithelial root sheath Present 4b
TS28: stratum intermedium Present 4b
TS28: stellate reticulum Absent 4b
Specimen 4d: postnatal day 7 (more )
Structure Level Pattern Image Note
TS28: molar enamel organ Present Regionally restricted 4d Expression is in Hertwig's epithelial root sheath and the stratum intermedium.
TS28: Hertwig's epithelial root sheath Present 4d
TS28: stratum intermedium Present 4d
Specimen 5c: embryonic day 13.0 (more )
Structure Level Pattern Image Note
TS21: incisor epithelium Present 5c
Specimen 5f: embryonic day 14.0 (more )
Structure Level Pattern Image Note
TS22: incisor enamel organ Present Regionally restricted 5f Expression is in the inner enamel epithelium, absent in the outer enamel epithelium.
TS22: inner enamel epithelium Present 5f
TS22: outer enamel epithelium Absent 5f
Specimen 5i: embryonic day 16.0 (more )
Note: (8d) is a higher magnification of the boxed area in (5i).
Structure Level Pattern Image Note
TS24: incisor enamel organ Very strong Regionally restricted 8d , 5i Expression is in the labial apical bud, stratum intermedium, and invaginated inner enamel epithelium, and in the transit-amplifying cell region, and is absent in the outer enamel epithelium, basal epithelium, and stellate reticulum of the labial apical buds.
TS24: inner enamel epithelium Very strong 5i , 8d
TS24: stratum intermedium Very strong 5i , 8d
TS24: outer enamel epithelium Absent 8d , 5i
Specimen 5k: embryonic day 18.0 (more )
Note: (8e) is a higher magnification of the boxed area in (5k). (k-1 inset) is a higher magnification of the boxed are (k-1).
Structure Level Pattern Image Note
TS26: incisor enamel organ Very strong Regionally restricted 5k , 8e Expression is in the labial apical bud, stratum intermedium, and invaginated inner enamel epithelium, and the transit-amplifying cell region, and is absent in the outer enamel epithelium, basal epithelium, and stellate reticulum of the labial apical buds.
TS26: inner enamel epithelium Very strong 8e , 5k
TS26: stratum intermedium Very strong 8e , 5k
TS26: outer enamel epithelium Absent 8e , 5k
TS26: stellate reticulum Absent 8e , 5k
Specimen 5m: postnatal day 7 (more )
Note: (8f) is a higher magnification of the boxed area in (5m). (m-1 inset) is a higher magnification of the boxed are (m-1).
Structure Level Pattern Image Note
TS28: incisor enamel organ Present Regionally restricted 5m , 8f Expression is in the labial apical bud and in the papillary layer adjacent to the mature enamel and in the transit-amplifying cell region, and is absent in the outer enamel epithelium, basal epithelium, and stellate reticulum of the labial apical buds.
TS28: outer enamel epithelium Absent 5m , 8f
TS28: stellate reticulum Absent 5m , 8f
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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