Antibody:	Anti-Lrp
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS11: primary trophoblast giant cell	Strong	Regionally restricted	4C	Strong staining was observed in the primary giant invading trophoblast cells at the anti-mesometrial pole.

Structure	Level	Pattern	Image	Note
TS11: trophoblast giant cell	Present		5A	Expression was detected in the invading trophoblast cells at the anti-mesometrial pole. Expression was absent from maternal cells.

Structure	Level	Pattern	Image	Note
TS11: ectoplacental cone	Present		5E	Expression was detected in the secondary trophoblast cells at the ectoplacental cone.
TS11: trophoblast giant cell	Present		5E	Expression was detected in the secondary trophoblast cells at the ectoplacental cone.

Structure	Level	Pattern	Image	Note
TS9: trophoblast giant cell	Weak			Expression was similar, albeit much weaker, to staining at E7.5.