About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:194507 Arango NA, et al., Meiosis I arrest abnormalities lead to severe oligozoospermia in meiosis 1 arresting protein (m1ap)-deficient mice. Biol Reprod. 2013;88(3):76
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5800493
Gene symbol: Gcna
Gene name: germ cell nuclear acidic protein
Antibody: anti-GCNA
Detection system: Secondary antibody coupled to Cy3
Results
Specimen 3A +/+: postnatal day 14 (more )
Note: Double labeled: red - Gcna; green - Ddx4 (assay MGI:5800481).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3A +/+ Expression was strong in spermatogonia and early spermatocytes and was more weak in later spermatocytes.
Specimen 3A M1aplz/lz: postnatal day 14; M1apGt(RRO290)Byg/M1apGt(RRO290)Byg (more )
Note: Double labeled: red - Gcna; green - Ddx4 (assay MGI:5800481).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Non-Uniform 3A M1aplz/lz There was a decrease in labeled cells. Some tubules appeared to be devoid of meiotic germ cells.
Specimen 3B +/+: postnatal day 21 (more )
Note: Double labeled: red - Gcna; green - Ddx4 (assay MGI:5800481).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3B +/+ Expression was strong in spermatogonia and early spermatocytes and was more weak in later spermatocytes.
Specimen 3B M1aplz/lz: postnatal day 21; M1apGt(RRO290)Byg/M1apGt(RRO290)Byg (more )
Note: Double labeled: red - Gcna; green - Ddx4 (assay MGI:5800481).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Non-Uniform 3B M1aplz/lz There was an abnormal distribution of immunopositive cells.
Specimen 3C +/+: postnatal day 28 (more )
Note: Double labeled: red - Gcna; green - Ddx4 (assay MGI:5800481).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3C +/+ Expression was strong in spermatogonia and early spermatocytes and was more weak in later spermatocytes.
Specimen 3C M1aplz/lz: postnatal day 28; M1apGt(RRO290)Byg/M1apGt(RRO290)Byg (more )
Note: Double labeled: red - Gcna; green - Ddx4 (assay MGI:5800481).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Non-Uniform 3C M1aplz/lz There was abnormal localization of expressing cells.
Specimen 3D +/+: postnatal day 56 (more )
Note: Double labeled: red - Gcna; green - Ddx4 (assay MGI:5800481).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3D +/+ Expression was strong in spermatogonia and early spermatocytes and was more weak in later spermatocytes. Strongly labeled cells were present near the periphery of the tubule.
Specimen 3D M1aplz/lz: postnatal day 56; M1apGt(RRO290)Byg/M1apGt(RRO290)Byg (more )
Note: Double labeled: red - Gcna; green - Ddx4 (assay MGI:5800481).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Non-Uniform 3D M1aplz/lz Cells with strong immunolabeling were seen a few layers inside the adluminal compartment.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory