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Gene Expression Data
Assay Details
Assay
Reference: J:194507 Arango NA, et al., Meiosis I arrest abnormalities lead to severe oligozoospermia in meiosis 1 arresting protein (m1ap)-deficient mice. Biol Reprod. 2013;88(3):76
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5800481
Gene symbol: Ddx4
Gene name: DEAD box helicase 4
Antibody: Anti-Mvh
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen 3A +/+: postnatal day 14 (more )
Note: Double labeled: green - Ddx4; red - Gcna (assay MGI:5800493).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3A +/+ Expression was detected in spermatogonia and primary and secondary spermatocytes.
Specimen 3A M1aplz/lz: postnatal day 14; M1apGt(RRO290)Byg/M1apGt(RRO290)Byg (more )
Note: Double labeled: green - Ddx4; red - Gcna (assay MGI:5800493).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Non-Uniform 3A M1aplz/lz There was a decrease in labeled cells. Some tubules appeared to be devoid of meiotic germ cells.
Specimen 3B +/+: postnatal day 21 (more )
Note: Double labeled: green - Ddx4; red - Gcna (assay MGI:5800493).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3B +/+ Expression was detected in spermatogonia and primary and secondary spermatocytes.
Specimen 3B M1aplz/lz: postnatal day 21; M1apGt(RRO290)Byg/M1apGt(RRO290)Byg (more )
Note: Double labeled: green - Ddx4; red - Gcna (assay MGI:5800493).
Structure Level Pattern Image Note
TS28: seminiferous tubule Weak Non-Uniform 3B M1aplz/lz There was a dramatic decrease in immunopositive cells in the mutant.
Specimen 3C +/+: postnatal day 28 (more )
Note: Double labeled: green - Ddx4; red - Gcna (assay MGI:5800493).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3C +/+ Expression was detected in spermatogonia and primary and secondary spermatocytes.
Specimen 3C M1aplz/lz: postnatal day 28; M1apGt(RRO290)Byg/M1apGt(RRO290)Byg (more )
Note: Double labeled: green - Ddx4; red - Gcna (assay MGI:5800493).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Non-Uniform 3C M1aplz/lz There was a decrease in labeled cells.
Specimen 3D +/+: postnatal day 56 (more )
Note: Double labeled: green - Ddx4; red - Gcna (assay MGI:5800493).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present 3D +/+ Expression was detected in spermatogonia and primary and secondary spermatocytes.
Specimen 3D M1aplz/lz: postnatal day 56; M1apGt(RRO290)Byg/M1apGt(RRO290)Byg (more )
Note: Double labeled: green - Ddx4; red - Gcna (assay MGI:5800493).
Structure Level Pattern Image Note
TS28: seminiferous tubule Present Non-Uniform 3D M1aplz/lz Some tubules contained few immunopositive cells, whereas others were negative.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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