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Gene Expression Data
Assay Details
Assay
Reference: J:229524 Robert T, et al., The TopoVIB-Like protein family is required for meiotic DNA double-strand break formation. Science. 2016 Feb 26;351(6276):943-9
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5800304
Gene symbol: Rpa1
Gene name: replication protein A1
Antibody: Anti-RPA
Detection system: Secondary antibody
Results
Specimen 5A +/+ Leptotene: postnatal day 30 (more )
Note: Double labeled: red - Rpa1; green - Sycp3 (assay MGI:5800309). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present 5A +/+ Leptotene RPA , 5A +/+ Leptotene Merge
Specimen 5A +/+ Zygotene: postnatal day 30 (more )
Note: Double labeled: red - Rpa1; green - Sycp3 (assay MGI:5800309). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present 5A +/+ Zygotene RPA , 5A +/+ Zygotene Merge
Specimen 5A +/+ Pachytene: postnatal day 30 (more )
Note: Double labeled: red - Rpa1; green - Sycp3 (assay MGI:5800309). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Present 5A +/+ Pachytene RPA , 5A +/+ Pachytene Merge
Specimen 5A -/- Leptotene: postnatal day 30; Top6blem2Arte/Top6blem2Arte (more )
Note: Double labeled: red - Rpa1; green - Sycp3 (assay MGI:5800309). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Weak 5A -/- Leptotene Merge , 5A -/- Leptotene RPA
Specimen 5A -/- Zygotene Like: postnatal day 30; Top6blem2Arte/Top6blem2Arte (more )
Note: Double labeled: red - Rpa1; green - Sycp3 (assay MGI:5800309). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Weak 5A -/- Zygotene like Merge upper , 5A -/- Zygotene like RPA upper
Specimen 5A -/- Zygotene Like: postnatal day 30; Top6blem2Arte/Top6blem2Arte (more )
Note: Double labeled: red - Rpa1; green - Sycp3 (assay MGI:5800309). Meiotic nuclear spread from testis.
Structure Level Pattern Image Note
TS28: spermatocyte Weak 5A -/- Zygotene like RPA lower , 5A -/- Zygotene like Merge lower
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory