Smad1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:218065 Hayata T, et al., Dullard/Ctdnep1 Regulates Endochondral Ossification via Suppression of TGF-beta Signaling. J Bone Miner Res. 2015 Feb;30(2):318-29
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5796788
Gene symbol:	Smad1
Gene name:	SMAD family member 1

Antibody:	anti-phospho-Smad1/5/9 (9511)
Assay notes:	The antibody recognizes the phosphorylated form of the protein; Smad1, Smad5, and Smad9 are annotated. A TSA-biotin system was used for detection.

Results

Specimen S8A Digit WT: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: digit mesenchyme	Present		S8A Digit WT

Specimen S8A Digit KO: embryonic day 13.5; Ctdnep1^tm3Ryn/Ctdnep1^tm3Ryn, Tg(Prrx1-cre)1Cjt/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS21: digit mesenchyme	Weak		S8A Digit KO

Specimen S8A Humerus WT: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: humerus	Present	Regionally restricted	S8A Humerus WT	Expression is in the growth plate.

Specimen S8A Humerus KO: embryonic day 15.5; Ctdnep1^tm3Ryn/Ctdnep1^tm3Ryn, Tg(Prrx1-cre)1Cjt/0 (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS23: humerus	Weak	Regionally restricted	S8A Humerus KO	Expression is in the growth plate.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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