Gene Expression Data
Assay Details
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Assay
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Reference:
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J:100495
Howard B, et al., Identification of the scaramanga gene implicates Neuregulin3 in mammary gland specification. Genes Dev. 2005 Sep 1;19(17):2078-90
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5796674
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Gene symbol:
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Lef1
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Gene name:
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lymphoid enhancer binding factor 1
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Probe:
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Lef probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1A:
embryonic day 11.75; Nrg3ska/ Nrg3ska
(more  )
Note: 48 somites
Specimen
1A:
(close  )
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Genetic Background:
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A/J
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Mutant Allele(s):
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Nrg3ska/Nrg3ska
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Age:
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embryonic day 11.75
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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48 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: mammary bud |
Present |
Regionally restricted |
1A
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Expression in mammary buds 1 and 4. Mammary bud 3 has not formed in the A/J embryo.
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Specimen
1B:
embryonic day 11.75
(more )
Note: 48 somites
Specimen
1B:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.75
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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48 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: mammary bud |
Present |
Regionally restricted |
1B
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Expression in mammary buds 1, 3 and 4 in the B6 embryo.
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Specimen
1C:
embryonic day 12.5; Nrg3ska/ Nrg3ska
(more )
Note: 53 somites
Specimen
1C:
(close )
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Genetic Background:
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A/J
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Mutant Allele(s):
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Nrg3ska/Nrg3ska
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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53 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: mammary bud |
Present |
Regionally restricted |
1C
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Expression in mammary buds 1, 2 and 4. Mammary bud 3 is absent in the A/J embryo.
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Specimen
1D:
embryonic day 12.5
(more )
Note: 55 somites
Specimen
1D:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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55 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: mammary bud |
Present |
Regionally restricted |
1D
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Expression in mammary buds 2, 3 and 4 in the B6 embryo.
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Specimen
1E:
embryonic day 12.5; Nrg3ska/ Nrg3ska
(more )
Note: 54 somites
Specimen
1E:
(close )
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Genetic Background:
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A/J
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Mutant Allele(s):
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Nrg3ska/Nrg3ska
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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54 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: mammary bud |
Present |
Regionally restricted |
1E
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Expression in mammary bud 4. Presumptive supernumerary bud adjacent to mammary bud 4.
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Specimen
1E inset:
embryonic day 12.5; Nrg3ska/ Nrg3ska
(more )
Note: 54 somites
Specimen
1E inset:
(close )
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Genetic Background:
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A/J
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Mutant Allele(s):
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Nrg3ska/Nrg3ska
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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54 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: integumental system |
Present |
Regionally restricted |
1E inset
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Expression in ectopic cells and in a line of cells connecting mammary bud 4 to ectopic bud. Expression in line of cells connective mammary bud 4 to bud 5.
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Analysis Tools
