Gene Expression Data
Assay Details
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Assay
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Reference:
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J:226857
Yang Y, et al., Lhx9 gene expression during early limb development in mice requires the FGF signalling pathway. Gene Expr Patterns. 2015 Sep-Nov;19(1-2):45-51
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5795655
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Gene symbol:
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Lhx9
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Gene name:
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LIM homeobox protein 9
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Probe:
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Lhx9E1-5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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The probe used in this assay likely recognizes all isoforms.
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Results
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Specimen
1B FL:
embryonic day 11.5
(more  )
Specimen
1B FL:
(close  )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Strong |
Regionally restricted |
1B FL
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Expression was detected in the anterior and distal regions. Posterior staining did not completely extend back towards the body wall.
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Specimen
1B HL:
embryonic day 11.5
(more )
Specimen
1B HL:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud |
Strong |
Regionally restricted |
1B HL
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Expression was detected in the anterior and distal regions. Posterior staining did not completely extend back towards the body wall.
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Specimen
3A:
embryonic day 10.5
(more )
Specimen
3A:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Present |
Regionally restricted |
3A
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Expression was strongest in the anterior region of the limb bud and expanding distally and down the posterior regions. Expression did not completely reach the posterior limb boundary. There was no staining in the zone of polarizing activity (ZPA) compared with Shh staining. There was staining in the progress zone by comparison to Fgf10 staining.
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Analysis Tools