Gene Expression Data
Assay Details
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Assay
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Reference:
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J:206928
Piper M, et al., NFIB-Mediated Repression of the Epigenetic Factor Ezh2 Regulates Cortical Development. J Neurosci. 2014 Feb 19;34(8):2921-30
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5792915
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Gene symbol:
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Pax6
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Gene name:
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paired box 6
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Antibody:
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anti-Pax6 (AB2237)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Data was summarized in Fig. 1J.
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Results
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Specimen
1F/F':
embryonic day 16.0
(more  )
Specimen
1F/F':
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
1H/H':
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Nfibtm1Rmg/Nfibtm1Rmg
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: hippocampus |
Present |
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1H
,
1H'
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Expression in neural progenitor cells was comparable between wild-type and mutant brains. Significantly more positive cells in the mutant at rostral level.
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Specimen
1G/G':
embryonic day 16.0
(more )
Specimen
1G/G':
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
1I/I':
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Nfibtm1Rmg/Nfibtm1Rmg
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: hippocampus |
Present |
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1I'
,
1I
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Expression in neural progenitor cells was comparable between wild-type and mutant brains. Significantly more positive cells in the mutant at caudal level.
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Specimen
not shown:
embryonic day 14.0
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Nfibtm1Rmg/Nfibtm1Rmg
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hippocampus |
Present |
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Same as in the wild-type at rostral and caudal levels.
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Specimen
not shown:
embryonic day 18.0
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Nfibtm1Rmg/Nfibtm1Rmg
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: hippocampus |
Present |
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Expression in neural progenitor cells was comparable between wild-type and mutant brains. Significantly more positive cells in the mutant at rostral and caudal levels.
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Analysis Tools