Antibody:	Anti-NFIB
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: brain	Absent		1F

Structure	Level	Pattern	Image	Note
TS28: brain	Present		1G

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	2B , 2B'	Expressed in the roofplate.

Structure	Level	Pattern	Image	Note
TS21: cortical preplate	Present		2D , 2D'

Structure	Level	Pattern	Image	Note
TS23: neocortex	Present	Regionally restricted	2F' , 2F	Expressed in layer VI.
TS23: cerebral cortex marginal layer	Present		2F , 2F'
TS23: cortical subplate	Present		2F , 2F'

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex marginal zone	Present		2H , 2H'
TS25: cerebral cortex subventricular zone	Present		2H , 2H'
TS25: cerebral cortex ventricular zone	Present		2H , 2H'
TS25: cortical subplate	Present		2H , 2H'
TS25: neocortex	Present	Regionally restricted	2H' , 2H	Expressed in layer VI.

Structure	Level	Pattern	Image	Note
TS27: cortical layer IV	Present		3B' , 3B

Structure	Level	Pattern	Image	Note
TS28: cortical layer IV	Present		3D , 3D'

Structure	Level	Pattern	Image	Note
TS28: cortical layer IV	Present		3F' , 3F	Reduced expression.

Structure	Level	Pattern	Image	Note
TS28: piriform cortex	Absent		3H , 3H'
TS28: cerebral cortex	Present		3H' , 3H	Reduced expression.

Structure	Level	Pattern	Image	Note
TS21: neocortex	Present	Uniform	4B' , 4B

Structure	Level	Pattern	Image	Note
TS23: neocortex	Present	Uniform	4D , 4D'

Structure	Level	Pattern	Image	Note
TS25: neocortex	Present	Uniform	4F' , 4F

Structure	Level	Pattern	Image	Note
TS28: neocortex	Present	Non-Uniform	4H , 4H" , 4H'" , 4H'	A graded expression in layers II/IIl. No differences in the distribution over the rostrocaudal axis in deep layers.

Structure	Level	Pattern	Image	Note
TS28: neocortex	Present	Non-Uniform	4J , 4J'" , 4J" , 4J'	A graded expression in layers II/IIl. No differences in the distribution over the rostrocaudal axis in deep layers.

Structure	Level	Pattern	Image	Note
TS23: neocortex	Strong	Regionally restricted	8B	Expressed in the cingulate cortex.
TS23: cerebral cortex	Strong	Regionally restricted	8B	Expressed in the cingulate cortex, but not in the midline glial populations.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex	Present	Regionally restricted	8D	Increased expression in the indusium griseum, the glial wedge, and also in the subcallosal sling.

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	8F	Expressed in the indusium griseum, the glial wedge, and also in the subcallosal sling.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex ventricular zone	Present		8H
TS28: cerebral cortex	Present	Regionally restricted	8H	Expression at the midline was lower than NFIA expression.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex subventricular zone	Present		8J
TS28: cerebral cortex ventricular zone	Present		8J
TS28: cerebral cortex	Present	Regionally restricted	8J , 8J'	Expressed in the indusium griseum. Expression of NFIA was more dorsomedially than the NFIB-expressing cells.

Structure	Level	Pattern	Image	Note
TS20: prethalamus	Present		9B
TS20: optic chiasma	Absent		9B , 9B'
TS20: telencephalon	Present	Regionally restricted	9B	Expressed in the ventral thalamus. No expression in the amygdala.
TS20: thalamus	Present	Regionally restricted	9B	Expressed in posterior thalamus.
TS20: hypothalamus	Present		9B

Structure	Level	Pattern	Image	Note
TS21: prethalamus	Present		9D
TS21: optic chiasma	Absent		9D , 9D'
TS21: telencephalon	Present	Regionally restricted	9D	Expressed in the ventral thalamus. No expression in the amygdala.
TS21: thalamus	Present	Regionally restricted	9D	Expressed in posterior thalamus.
TS21: hypothalamus	Present		9D

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	10B , 10B'	Expressed in the hippocampal primordium.

Structure	Level	Pattern	Image	Note
TS23: hippocampus	Present	Regionally restricted	10D , 10D'	Expression was highest in the hippocampal stratum radiatum.

Structure	Level	Pattern	Image	Note
TS25: dentate gyrus	Present		10F , 10F'
TS25: hippocampus	Present	Regionally restricted	10F' , 10F	Expressed in the stratum oriens. No expression in the stratum radiatum.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Strong		11B'" , 11B , 11B' , 11B"
TS28: dentate gyrus granule cell layer	Strong		11B'" , 11B , 11B' , 11B"
TS28: CA1 pyramidal cell layer	Absent		11B' , 11B , 11B"
TS28: CA2 pyramidal cell layer	Absent		11B' , 11B , 11B"
TS28: CA3 pyramidal cell layer	Absent		11B' , 11B , 11B"

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Strong		11D' , 11D
TS28: dentate gyrus granule cell layer	Strong		11D' , 11D

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Strong		11F , 11F'
TS28: dentate gyrus granule cell layer	Strong		11F , 11F'

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Strong		11H" , 11H , 11H'" , 11H'
TS28: dentate gyrus granule cell layer	Strong		11H" , 11H , 11H'" , 11H'

Structure	Level	Pattern	Image	Note
TS27: thalamus	Absent