Probe:	tek cDNA2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS20: heart	Present		3a
TS20: primitive meninges	Present	Regionally restricted	3a	Expressed in the leptomeninges lining the brain and spinal cord.
TS20: blood vessel endothelium	Present		3a	Expressed in the inner lining of major blood vessels, including the caudal vein and basilar and renal arteries.
TS20: intersomitic artery	Present		3a	Thin band.
TS20: basilar artery	Present	Regionally restricted	3a	Expressed in the inner lining.

Structure	Level	Pattern	Image	Note
TS20: atrium endocardial lining	Present		3c , 3b
TS20: endocardial lining	Present		3c , 3b
TS20: liver vasculature	Present		3c , 3b
TS20: 4th arch artery	Present	Regionally restricted	3b , 3c	Expressed in the lining cells.
TS20: 6th arch artery	Present	Regionally restricted	3b , 3c	Expressed in the lining cells.
TS20: sinoatrial node	Present	Regionally restricted	3b , 3c	Expressed in the lining cells.
TS20: sinus venosus	Present	Regionally restricted	3b , 3c	Expressed in the lining cells.
TS20: blood vessel	Present		3c , 3b	Expressed in numerous small blood vessles perforating the liver and mandible.

Structure	Level	Pattern	Image	Note
TS13: amnion	Present	Regionally restricted	4b , 4a	Expressed in the mesodermal component.
TS13: yolk sac	Present	Regionally restricted	4b , 4a	Expressed in the mesodermal component.
TS13: endocardial tube	Present		4b , 4a
TS13: dorsal aorta	Present	Regionally restricted	4a , 4b	Expressed in the inner lining.
TS13: primary head vein	Present	Regionally restricted	4a , 4b	Expressed in the inner lining.

Structure	Level	Pattern	Image	Note
TS13: blood island	Present	Regionally restricted	4c	Expressed in flat, elongated cells of endothelial-like morphology.

Structure	Level	Pattern	Image	Note
TS13: head mesenchyme	Present	Regionally restricted	4f	Expressed in large, round cells that correspond to angioblasts, the presumptive progenitor of endothelial cells.

Structure	Level	Pattern	Image	Note
TS13: endocardial tube	Present	Regionally restricted	4h , 4i , 4g	Expressed in cells with endothelial- and angioblast-like morphology in the developing endocardium.
TS13: somite	Present	Regionally restricted	4h , 4j , 4g	Expressed in cells extending beneath, and possibly from , the ventral surface of the somites.
TS13: head mesenchyme	Present	Regionally restricted	4h , 4g	Expressed in regions thought to contain developing vasculature.

Structure	Level	Pattern	Image	Note
TS20: primitive meninges	Strong	Regionally restricted	6b	Expressed in the developing leptomeninges.

Structure	Level	Pattern	Image	Note
TS28: great vessel of heart	Present	Regionally restricted	7a	Expressed in endothelial cells lining the a rtery and vein.

Structure	Level	Pattern	Image	Note
TS28: artery endothelium	Present		7b

Structure	Level	Pattern	Image	Note
TS28: vein endothelium	Present		7c

Structure	Level	Pattern	Image	Note
TS9: ectoplacental cone	Absent
TS9: embryo	Absent

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted		Expressed in the inner lining of the small blood vessels and capillaries.

Structure	Level	Pattern	Image	Note
TS12: yolk sac	Present
TS12: head mesenchyme	Present	Clusters

Structure	Level	Pattern	Image	Note
TS28: endocardial lining	Present			Expression is lower than that observed in E12.5.
TS28: blood vessel endothelium	Present	Regionally restricted		Expressed in the endothelial lining of major blood vessels, both arteries and veins, connecting with the adult heart. Expression is lower than that observed in E12.5.
TS28: great vessel of heart	Present	Regionally restricted		Expressed in the endothelial lining of major blood vessels, both arteries and veins, connecting with the adult heart. Expression is lower than that observed in E12.5.