Gene Expression Data
Assay Details
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Assay
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Reference:
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J:231881
Zeng L, et al., Essential Roles of Cyclin Y-Like 1 and Cyclin Y in Dividing Wnt-Responsive Mammary Stem/Progenitor Cells. PLoS Genet. 2016 May;12(5):e1006055
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5790324
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Gene symbol:
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Axin2
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Gene name:
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axin 2
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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Fixative was 0.2% glutaraldehyde, 1.5% formaldehyde.
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Results
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Specimen
2D:
postnatal week 5; Axin2tm1Wbm/Axin2 +
(more  )
Note: Right panel is a higher magnification of the boxed region.
Specimen
2D:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Axin2tm1Wbm/Axin2+
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Age:
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postnatal week 5
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Right panel is a higher magnification of the boxed region.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: mammary gland |
Present |
Regionally restricted |
2D
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Expression is in the terminal end buds.
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Specimen
2E:
postnatal week 6; Axin2tm1Wbm/Axin2 +
(more )
Note: Right panel is a higher magnification of the boxed region.
Specimen
2E:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Axin2tm1Wbm/Axin2+
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Age:
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postnatal week 6
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Sex:
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Female
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Type:
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whole mount
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Fixation:
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Other - See Notes
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Note:
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Right panel is a higher magnification of the boxed region.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: mammary gland |
Present |
Regionally restricted |
2E
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Expression is in the terminal end buds.
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Specimen
2F:
postnatal week 5; Axin2tm1Wbm/Axin2 +
(more )
Specimen
2F:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Axin2tm1Wbm/Axin2+
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Age:
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postnatal week 5
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Sex:
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Female
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: mammary gland |
Present |
Regionally restricted |
2F
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Expression is in the terminal end buds in basal cells and surrounding stromal cells.
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Specimen
S3B:
postnatal week 8; Axin2tm1Wbm/Axin2 +
(more )
Specimen
S3B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Axin2tm1Wbm/Axin2+
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Age:
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postnatal week 8
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Sex:
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Female
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Analysis Tools