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Gene Expression Data
Assay Details
Assay
Reference: J:198275 Watanabe N, et al., The REV7 subunit of DNA polymerase zeta is essential for primordial germ cell maintenance in the mouse. J Biol Chem. 2013 Apr 12;288(15):10459-71
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5789138
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: Anti-Oct4
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 5C E9.5 Rev7+/+: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: primordial germ cell of hindgut Present 5C Oct4 Rev7+/+
Specimen 5C E9.5 Rev7-/-: embryonic day 9.5; Mad2l2tm1Ymu/Mad2l2tm1Ymu (more )
Structure Level Pattern Image Note
TS15: primordial germ cell of hindgut Present 5C Oct4 Rev7-/- Costaining with TUNEL indicated some primordial germ cells in the hindgut region were double stained in the mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
MGI 6.24 		The Jackson Laboratory