Casp3 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:198275 Watanabe N, et al., The REV7 subunit of DNA polymerase zeta is essential for primordial germ cell maintenance in the mouse. J Biol Chem. 2013 Apr 12;288(15):10459-71
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5789136
Gene symbol:	Casp3
Gene name:	caspase 3

Antibody:	anti-cleaved Caspase 3
Detection system:	Secondary antibody coupled to horseradish peroxidase

Results

Specimen 5B E11.5 Rev7+/+: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: urogenital ridge	Ambiguous		5B Casp3 E11.5 Rev7+/+

Specimen 5B E11.5 Rev7-/-: embryonic day 11.5; Mad2l2^tm1Ymu/Mad2l2^tm1Ymu (more

)

Structure	Level	Pattern	Image	Note
TS19: urogenital ridge	Present		5B Casp3 E11.5 Rev7-/-	Immunopositive cells were detected at a high frequency around the genital ridges of the mutant compared to the wild type.

6A Rev7+/+ ©

Specimen 6A Rev7+/+: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: forebrain	Ambiguous		6A Rev7+/+

6A Rev7-/- ©

Specimen 6A Rev7-/-: embryonic day 11.5; Mad2l2^tm1Ymu/Mad2l2^tm1Ymu (more

)

Structure	Level	Pattern	Image	Note
TS19: forebrain	Present		6A Rev7-/-	Immunopositive apoptotic cells were detected among neuroblasts in the forebrain. The frequency of apoptotic cells was significantly increased in the mutant embryos compared to wild type.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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