Cdh1 In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:96963 Stemmler MP, et al., E-cadherin intron 2 contains cis-regulatory elements essential for gene expression. Development. 2005 Mar;132(5):965-76
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:5784500
Gene symbol:	Cdh1
Gene name:	cadherin 1

Reporter:	lacZ
	Direct Detection
Assay notes:	Fixative was 1% formaldehyde/0.2% glutaraldehyde and a postfixation with 4% paraformaldehyde.

Results

3A ©

Specimen 3A: embryonic day 6.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS9: extraembryonic ectoderm	Strong		3A
TS9: embryo ectoderm	Present		3A

3B ©

Specimen 3B: embryonic day 7.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Image
TS11: embryo ectoderm	Present	3B
TS11: embryo endoderm	Present	3B
TS11: embryo mesoderm	Absent	3B

3C ©

Specimen 3C: embryonic day 8.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Image
TS13: gut endoderm	Very strong	3C
TS13: surface ectoderm	Present	3C
TS13: yolk sac	Present	3C

3D ©

Specimen 3D: embryonic day 9.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Image
TS15: gut endoderm	Very strong	3D
TS15: surface ectoderm	Present	3D
TS15: yolk sac	Present	3D

3E ©

Specimen 3E: embryonic day 10.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Image
TS17: gut endoderm	Very strong	3E
TS17: yolk sac	Strong	3E
TS17: surface ectoderm	Present	3E

3F ©

Specimen 3F: embryonic day 7.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Image	Note
TS11: embryo endoderm	Present	3F
TS11: embryo ectoderm	Present	3F	Expression levels appear higher in posterior ectoderm.
TS11: embryo mesoderm	Absent	3F

3G ©

Specimen 3G: embryonic day 10.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Image
TS17: gut epithelium	Strong	3G
TS17: pharynx	Strong	3G
TS17: gut endoderm	Present	3G

3H ©

Specimen 3H: embryonic day 6.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS9: embryo	Absent		3H
TS9: extraembryonic ectoderm	Absent		3H

3I ©

Specimen 3I: embryonic day 7.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Image
TS11: embryo	Absent	3I
TS11: embryo ectoderm	Absent	3I
TS11: embryo endoderm	Absent	3I

3J ©

Specimen 3J: embryonic day 8.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent		3J
TS13: gut endoderm	Absent		3J

3K ©

Specimen 3K: embryonic day 9.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS15: embryo	Absent		3K
TS15: gut endoderm	Absent		3K

3L ©

Specimen 3L: embryonic day 10.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Image
TS17: yolk sac	Present	3L
TS17: forelimb bud apical ectodermal ridge	Weak	3L
TS17: gut endoderm	Absent	3L

3M ©

Specimen 3M: embryonic day 7.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Image
TS11: embryo	Absent	3M
TS11: embryo ectoderm	Absent	3M
TS11: embryo endoderm	Absent	3M

3N ©

Specimen 3N: embryonic day 10.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS17: gut endoderm	Absent		3N

Specimen not shown: embryonic day 11.0; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS18: branchial arch	Present	Regionally restricted		Expression is between the first and second branchial arch.

4A ©

Specimen 4A: embryonic day 11.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)
Note: Overnight staining for B-gal expression.

Structure	Level	Pattern	Image	Note
TS19: surface ectoderm	Strong		4A

4B ©

Specimen 4B: embryonic day 12.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)
Note: Overnight staining for B-gal expression.

Structure	Level	Image
TS20: face skin	Present	4B
TS20: skin	Present	4B
TS20: vibrissa follicle	Present	4B

4C ©

Specimen 4C: embryonic day 16.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)
Note: 45 minute staining for B-gal expression.

Structure	Level	Pattern	Image	Note
TS24: skin	Strong		4C

4D ©

Specimen 4D: embryonic day 16.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)
Note: 45 minute staining for B-gal expression.

Structure	Level	Image
TS24: gut	Strong	4D
TS24: pancreas	Strong	4D
TS24: skin	Strong	4D
TS24: stomach	Strong	4D
TS24: thymus	Strong	4D

4E ©

Specimen 4E: embryonic day 16.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)
Note: Isolated organs. Overnight staining for B-gal expression.

Structure	Level	Image
TS24: gut	Strong	4E
TS24: pancreas	Strong	4E
TS24: stomach	Strong	4E
TS24: thymus	Strong	4E

4F ©

Specimen 4F: embryonic day 11.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)
Note: Overnight staining for B-gal expression.

Structure	Level	Pattern	Image	Note
TS19: surface ectoderm	Weak	Regionally restricted	4F	Expression is in the facial region.
TS19: branchial arch ectoderm	Weak	Regionally restricted	4F	Expression is in the mandibular and maxillary components.
TS19: apical ectodermal ridge	Weak		4F

4G ©

Specimen 4G: embryonic day 12.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)
Note: Overnight staining for B-gal expression.

Structure	Level	Pattern	Image	Note
TS20: face skin	Weak		4G
TS20: vibrissa follicle	Weak		4G

4H ©

Specimen 4H: embryonic day 16.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)
Note: 45 minute staining for B-gal expression.

Structure	Level	Pattern	Image	Note
TS24: skin	Present		4H

4I ©

Specimen 4I: embryonic day 16.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)
Note: 45 minute staining for B-gal expression.

Structure	Level	Image
TS24: skin	Present	4I
TS24: esophagus	Weak	4I
TS24: pancreas	Weak	4I

4J ©

Specimen 4J: embryonic day 16.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)
Note: Isolated organs. Overnight staining for B-gal expression.

Structure	Level	Image
TS24: lung epithelium	Weak	4J
TS24: esophagus	Present	4J
TS24: pancreas	Present	4J

4K ©

Specimen 4K: embryonic day 11.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Strong		4K
TS19: head surface ectoderm	Strong	Regionally restricted	4K	Expression is in the ectoderm surrounding the eye.

4L ©

Specimen 4L: embryonic day 11.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: surface ectoderm	Present		4L

4M ©

Specimen 4M: embryonic day 11.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: nasal pit	Present		4M

4N ©

Specimen 4N: embryonic day 11.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: stomach	Present		4N

4O ©

Specimen 4O: embryonic day 11.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: pancreas primordium	Present		4O

4P ©

Specimen 4P: embryonic day 11.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: gut	Present		4P

4Q ©

Specimen 4Q: embryonic day 11.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: metanephros	Present		4Q

4R ©

Specimen 4R: embryonic day 11.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: head surface ectoderm	Absent		4R
TS19: lens vesicle	Absent		4R

4S ©

Specimen 4S: embryonic day 11.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: surface ectoderm	Trace	Regionally restricted	4S	Shown is an area where expression is completely absent (back skin). Very faint expression is seen in the facial region and at the apical ectodermal ridge.

4T ©

Specimen 4T: embryonic day 11.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: nasal pit	Absent		4T

4U ©

Specimen 4U: embryonic day 11.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: stomach	Absent		4U

4V ©

Specimen 4V: embryonic day 11.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: pancreas primordium	Weak		4V

4W ©

Specimen 4W: embryonic day 11.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: gut	Absent		4W

4X ©

Specimen 4X: embryonic day 11.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS19: metanephros	Absent		4X

5B +/flox ©

Specimen 5B +/flox: embryonic day 10.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS17: yolk sac	Strong		5B +/flox

5B +/del ©

Specimen 5B +/del: embryonic day 10.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS17: yolk sac	Strong		5B +/del

5D +/flox ©

Specimen 5D left: embryonic day 12.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS20: yolk sac	Strong		5D +/flox

5D +/del ©

Specimen 5D right: embryonic day 12.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS20: yolk sac	Strong		5D +/del

6A ©

Specimen 6A: embryonic day 10.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS17: lens vesicle	Very strong		6A

6B ©

Specimen 6B: embryonic day 10.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS17: lens vesicle	Absent		6B

6C ©

Specimen 6C: embryonic day 12.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS20: lens vesicle	Very strong		6C

6D ©

Specimen 6D: embryonic day 12.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS20: lens vesicle	Absent		6D

6E ©

Specimen 6E: embryonic day 14.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS22: lens	Very strong		6E

6F ©

Specimen 6F: embryonic day 14.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS22: lens	Absent		6F

6G ©

Specimen 6G: embryonic day 16.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: salivary gland	Strong		6G

6H ©

Specimen 6H: embryonic day 16.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: salivary gland	Absent		6H

6I ©

Specimen 6I: embryonic day 16.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: salivary gland	Strong		6I

6J ©

Specimen 6J: embryonic day 16.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: thyroid gland	Present		6J

6K ©

Specimen 6K: embryonic day 16.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: trachea	Present		6K

6L ©

Specimen 6L: embryonic day 16.5; Cdh1^tm3.1Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: meninges	Present		6L

6M ©

Specimen 6M: embryonic day 16.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: salivary gland	Absent		6M

6N ©

Specimen 6N: embryonic day 16.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: thyroid gland	Weak		6N

6O ©

Specimen 6O: embryonic day 16.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: trachea	Weak		6O

6P ©

Specimen 6P: embryonic day 16.5; Cdh1^tm3.2Kem/Cdh1⁺ (more

)

Structure	Level	Pattern	Image	Note
TS24: meninges	Weak		6P

S2I ©

Specimen S2 I: embryonic day 5.5; Cdh1^tm6Kem/Cdh1^tm6Kem (more

)

Structure	Level	Pattern	Image	Note
TS8: conceptus	Present		S2I

S2J ©

Specimen S2 J: embryonic day 6.5; Cdh1^tm6Kem/Cdh1^tm6Kem (more

)

Structure	Level	Pattern	Image	Note
TS9: extraembryonic ectoderm	Present		S2J

S2K ©

Specimen S2 K: embryonic day 7.5; Cdh1^tm6Kem/Cdh1^tm6Kem (more

)

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present		S2K

S2L ©

Specimen S2 L: embryonic day 8.5; Cdh1^tm6Kem/Cdh1^tm6Kem (more

)

Structure	Level	Pattern	Image	Note
TS13: gut endoderm	Present		S2L

S2M ©

Specimen S2 M: embryonic day 9.5; Cdh1^tm6Kem/Cdh1^tm6Kem (more

)

Structure	Level	Pattern	Image	Note
TS15: gut endoderm	Present		S2M
TS15: surface ectoderm	Present		S2M

S2N ©

Specimen S2 N: embryonic day 10.5; Cdh1^tm6Kem/Cdh1^tm6Kem (more

)

Structure	Level	Image
TS17: gut endoderm	Present	S2N
TS17: lens vesicle	Present	S2N
TS17: surface ectoderm	Present	S2N

S2O ©

S2O inset ©

Specimen S2 O: embryonic day 11.5; Cdh1^tm6Kem/Cdh1^tm6Kem (more

)
Note: Inset shows a higher magnification of the ventral midbrain.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present		S2O , S2O inset	Expression is in the ventral midbrain.

S2P ©

Specimen S2 P: embryonic day 12.5; Cdh1^tm6Kem/Cdh1^tm6Kem (more

)

Structure	Level	Pattern	Image	Note
TS20: skin	Present		S2P

S2Q ©

Specimen S2 Q: embryonic day 14.5; Cdh1^tm6Kem/Cdh1^tm6Kem (more

)

Structure	Level	Pattern	Image	Note
TS22: hair follicle	Present		S2Q

S2R ©

Specimen S2 R: embryonic day 16.5; Cdh1^tm6Kem/Cdh1^tm6Kem (more

)

Structure	Level	Pattern	Image	Note
TS24: skin	Present		S2R

S2S ©

Specimen S2 S: embryonic day 14.5; Cdh1^tm6Kem/Cdh1^tm6Kem (more

)

Structure	Level	Pattern	Image	Note
TS22: gut epithelium	Present		S2S

S2T ©

Specimen S2 T: embryonic day 14.5; Cdh1^tm6Kem/Cdh1^tm6Kem (more

)

Structure	Level	Pattern	Image	Note
TS22: gut epithelium	Present		S2T

S2U ©

Specimen S2 U: embryonic day 7.5; Cdh1^tm6Kem/Cdh1^tm6Kem (more

)

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present		S2U

S2V ©

Specimen S2 V: embryonic day 7.5; Cdh1^tm6Kem/Cdh1^tm6Kem (more

)

Structure	Level	Pattern	Image	Note
TS11: embryo ectoderm	Present		S2V

S2W ©

Specimen S2 W: embryonic day 8.0; Cdh1^tm6Kem/Cdh1^tm6Kem (more

)

Structure	Level	Pattern	Image	Note
TS12: embryo	Present		S2W

S2X ©

Specimen S2 X: embryonic day 8.0; Cdh1^tm6Kem/Cdh1^tm6Kem (more

)

Structure	Level	Pattern	Image	Note
TS12: embryo	Present		S2X

S2Y ©

Specimen S2 Y: embryonic day 11.5; Cdh1^tm6Kem/Cdh1^tm6Kem (more

)

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	S2Y	Expression is in the ventral midbrain.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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