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Gene Expression Data
Assay Details
Assay
Reference: J:90073 Miyoshi G, et al., Identification of a novel basic helix-loop-helix gene, Heslike, and its role in GABAergic neurogenesis. J Neurosci. 2004 Apr 7;24(14):3672-82
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5780718
Gene symbol: Gad2
Gene name: glutamic acid decarboxylase 2
Antibody: anti-GAD65
Detection system: Secondary antibody
Results
Specimen 5B: embryonic day 9.5 (more )
Note: Double labeled: green - GAD65 (Gad2); red - Heslike (Helt; assay MGI:5780714).
Structure Level Pattern Image Note
TS15: future midbrain Absent 5B GABAergic neurons are not yet formed.
Specimen 5E: embryonic day 10.5 (more )
Note: Double labeled: green - GAD65 (Gad2); red - Heslike (Helt; assay MGI:5780714).
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 5E/E' Expression in neurons in mantle layer just outside the ventricular zone
Specimen 5H: embryonic day 11.5 (more )
Note: Double labeled: green - GAD65 (Gad2); red - Heslike (Helt; assay MGI:5780714).
Structure Level Pattern Image Note
TS19: midbrain Present Regionally restricted 5H/H' Region for expression in neurons is expanded dorsally.
Specimen 5K: embryonic day 12.5 (more )
Note: Double labeled: green - GAD65 (Gad2); red - Heslike (Helt; assay MGI:5780714).
Structure Level Pattern Image Note
TS20: midbrain Present Regionally restricted 5K/K' Further dorsal expansion of region of expression in neurons.
Specimen 6B: embryonic day 10.5 (more )
Note: Double labeled: green - GAD65 (Gad2); red - Heslike (Helt; assay MGI:5780714).
Structure Level Pattern Image Note
TS17: diencephalon Present Regionally restricted 6B Expression in neurons in the mantle layer just outside Helt-expressing ventricular regions in the pretectum.
Specimen 6F: embryonic day 11.5 (more )
Note: Double labeled: green - GAD65 (Gad2); red - Heslike (Helt; assay MGI:5780714).
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted 6F Expression in neurons formed in the mantle layer just outside Helt-expressing ventricular zone.
Specimen 6J: embryonic day 12.5 (more )
Note: Double labeled: green - GAD65 (Gad2); red - Heslike (Helt; assay MGI:5780714).
Structure Level Pattern Image Note
TS20: diencephalon Present Regionally restricted 6J Expression in neurons formed in the mantle layer just outside Helt-expressing ventricular zone.
Specimen 6M: embryonic day 11.5 (more )
Note: Double labeled: green - GAD65 (Gad2); red - Heslike (Helt; assay MGI:5780714).
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted 6M Expressing cells just outside Helt-expressing region near the zona limitans intrathalamica.
Specimen 7A: embryonic day 10.5 (more )
Note: Double labeled: green - GAD65 (Gad2); red - Heslike (Helt; assay MGI:5780714).
Structure Level Pattern Image Note
TS17: midbrain Present Regionally restricted 7A
Specimen 7I: embryonic day 11.5 (more )
Note: Double labeled: green - GAD65 (Gad2); red - Heslike (Helt; assay MGI:5780714).
Structure Level Pattern Image Note
TS19: midbrain Present Regionally restricted 7I
Specimen 7M: embryonic day 11.5 (more )
Note: Double labeled: green - GAD65 (Gad2); red - Heslike (Helt; assay MGI:5780714).
Structure Level Pattern Image Note
TS19: diencephalon Present Regionally restricted 7M Expression in caudal diencephalon.
Specimen 8C: embryonic day 11.5 (more )
Note: Double labeled: green - GAD65 (Gad2); red - Heslike (Helt; assay MGI:5780714).
Structure Level Pattern Image Note
TS19: midbrain Present Regionally restricted 8C/C' Expression in many neurons in the mantle layer.
Specimen 8D: embryonic day 11.5; Ascl1tm1And/Ascl1tm1And (more )
Note: Double labeled: green - GAD65 (Gad2); red - Heslike (Helt; assay MGI:5780714).
Structure Level Pattern Image Note
TS19: midbrain Ambiguous 8D/D' Authors report virtually no expression in the mutant mesencephalon.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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