Gene Expression Data
Assay Details
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Assay
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Reference:
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J:31833
Godin I, et al., TGF beta 1 inhibits proliferation and has a chemotropic effect on mouse primordial germ cells in culture. Development. 1991 Dec;113(4):1451-7
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5779848
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Gene symbol:
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Tgfb1
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Gene name:
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transforming growth factor, beta 1
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Antibody:
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anti-CC (1-30)
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Detection system:
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Secondary antibody coupled to fluorescein
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Assay notes:
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Fixation with 2% trichloroacetic acid.
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Results
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Specimen
3D:
embryonic day 10.5
(more  )
Specimen
3D:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: body wall mesenchyme |
Strong |
Regionally restricted |
3D
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Strong expression in mesenchymal tissues in the dorsal body wall of the embryos.
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| TS17: gut |
Present |
Regionally restricted |
3D
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Partial expression in the gut, concentrated in dorsal-most parts.
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| TS17: central nervous system |
Absent |
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3D
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No expression in epithelial tissues, including the central nervous system, mesonephric ducts, hindgut and epidermis.
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| TS17: hindgut |
Absent |
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3D
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No expression in epithelial tissues, including the central nervous system, mesonephric ducts, hindgut and epidermis.
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| TS17: nephric duct |
Absent |
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3D
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No expression in epithelial tissues, including the central nervous system, mesonephric ducts, hindgut and epidermis.
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| TS17: surface ectoderm |
Absent |
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3D
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No expression in epithelial tissues, including the central nervous system, mesonephric ducts, hindgut and epidermis.
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Specimen
3E:
embryonic day 10.5
(more )
Specimen
3E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: gut mesentery |
Weak |
Regionally restricted |
3E
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In more caudal regions, little expression in anypart of gut mesentery.
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Specimen
3F:
embryonic day 10.5
(more )
Specimen
3F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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Specimen
3G:
embryonic day 10.5
(more )
Specimen
3G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud |
Weak |
Regionally restricted |
3G
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Small scattered foci of expression seen between cells at high magnification.
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Analysis Tools