Probe:	DP1 probe
Probe preparation:	labelled with P32 DNA
Visualized with:	Autoradiography
Assay notes:	Two probes/antibodies were described, but the one used in this assay was not detailed. Probe/antibody name MGI:1097406 may have been used in this assay.

Structure	Level	Pattern	Image	Note
TS21: pons	Present		6I/J
TS21: brain ventricular layer	Present	Widespread	6I/J	Expression somewhat more abundant in ventricular layers throughout the developing brain.

Structure	Level	Pattern	Image	Note
TS19: brain	Present	Widespread	6K/L	Expression throughout the brain.

Structure	Level	Pattern	Image	Note
TS21: 4th ventricle choroid plexus	Absent		8E

Structure	Level	Pattern	Image	Note
TS22: lateral ventricle choroid plexus	Absent		8F/G
TS22: brain	Present		8F/G	Expression in neighboring brain tissue.

Structure	Level	Pattern	Image	Note
TS17: brain	Present

Structure	Level	Pattern	Image	Note
TS19: central nervous system	Present	Widespread
TS19: peripheral nervous system	Present	Widespread
TS19: brain marginal layer	Present
TS19: brain ventricular layer	Present
TS19: neural retina	Present	Regionally restricted		Expression in neuroblastic layer.
TS19: heart	Present
TS19: liver	Present

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present
TS20: lung	Present
TS20: rib pre-cartilage condensation	Strong	Regionally restricted		Between 12.5 and 19.5 dpc, high levels of expression in areas corresponding to proliferating chondrocytes.
TS20: vertebral cartilage condensation	Strong	Regionally restricted		Between 12.5 and 19.5 dpc, high levels of expression in areas corresponding to proliferating chondrocytes.

Structure	Level	Pattern	Image	Note
TS21: metanephros	Present

Structure	Level	Pattern	Image	Note
TS22: neural retina	Present	Regionally restricted		Expression in both retinoblast and ganglion cell layers.

Structure	Level	Pattern	Image	Note
TS23: thymus primordium	Very strong	Patchy		Very high levels of expression present from 15.5 to 19.5 dpc.
TS23: skeletal muscle	Weak

Structure	Level	Pattern	Image	Note
TS26: central nervous system	Present	Widespread
TS26: peripheral nervous system	Present	Widespread
TS26: spinal cord marginal layer	Present
TS26: spinal cord ventricular layer	Present
TS26: dorsal root ganglion	Present
TS26: neural retina	Present	Regionally restricted		Expression in both retinoblast and ganglion cell layers.
TS26: bone	Absent

Structure	Level	Pattern	Image	Note
TS26: brain marginal layer	Present
TS26: brain ventricular layer	Present
TS26: choroid plexus	Absent
TS26: thymus	Very strong			Very high levels of expression present from 15.5 to 19.5 dpc.
TS26: heart	Present
TS26: liver	Present
TS26: lung	Present
TS26: metanephros	Present
TS26: skeletal muscle	Weak
TS26: rib cartilage	Strong	Regionally restricted		Between 12.5 and 19.5 dpc, high levels of expression in areas corresponding to prolferating chondrocytes.
TS26: vertebral cartilage condensation	Strong	Regionally restricted		Between 12.5 and 19.5 dpc, high levels of expression in areas corresponding to prolferating chondrocytes.
TS26: bone	Absent

Structure	Level	Pattern	Image	Note
TS28: retina nuclear layer	Present			Expression in both retinoblast and ganglion cell layers.
TS28: retina ganglion cell layer	Strong			Higher levels of expression in differentiating cell layers than in retinoblasts.
TS28: retina layer	Present	Widespread		Expression in all retinal layers.