Probe:	mE2F-2-D2
Probe preparation:	labelled with P32 DNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present		4A	Expression first detected in forebrain.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Widespread	4B	Expression in midbrain and hindbrain.
TS17: midbrain	Present	Widespread	4B	Expression in midbrain and hindbrain.
TS17: future spinal cord	Weak	Regionally restricted	4B	Lower levels in upper regions of the spinal cord.

Structure	Level	Pattern	Image	Note
TS19: brain	Present		4C	Expression throughout the developing brain.

Structure	Level	Pattern	Image	Note
TS21: forebrain ventricular layer	Present		4D/E	Peak expression at 12.5-13.5 dpc.

Structure	Level	Pattern	Image	Note
TS21: spinal cord	Strong	Regionally restricted	4F/G	Abundant expression in the dorsal region, but not ventral region.
TS21: vertebral cartilage condensation	Present		4F/G

Structure	Level	Pattern	Image	Note
TS21: 4th ventricle choroid plexus	Trace		8B	Expression in posterior choroid plexus.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Absent			Broad expression in brain.
TS15: future midbrain	Absent			Broad expression in brain.
TS15: future spinal cord	Absent			Broad expression in brain.

Structure	Level	Pattern	Image	Note
TS17: brain	Present	Widespread		Broad expression in brain.

Structure	Level	Pattern	Image	Note
TS19: brain ventricular layer	Present
TS19: brain marginal layer	Absent			No expression in intermediate or marginal layers.
TS19: neural retina	Present	Regionally restricted		Expression in neuroblastic layer.
TS19: heart	Present
TS19: liver	Present

Structure	Level	Pattern	Image	Note
TS20: brain ventricular layer	Present	Widespread		Broad expression in undifferentiated ventricular regions of the brain.
TS20: spinal cord ventricular layer	Present
TS20: spinal cord marginal layer	Absent
TS20: dorsal root ganglion	Absent
TS20: lung	Present
TS20: cartilage condensation	Weak

Structure	Level	Pattern	Image	Note
TS21: choroid plexus	Trace			Expression is barely above background.

Structure	Level	Pattern	Image	Note
TS22: neural retina	Strong	Regionally restricted		Very abundant expression in embryonic retinoblastic cell layer. No expression in differentiated ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS23: brain ventricular layer	Present	Widespread		Broad expression in undifferentiated ventricular regions of the brain.
TS23: thymus primordium	Very strong			Very high levels of expression present from 15.5 to 19.5 dpc.
TS23: heart	Present

Structure	Level	Pattern	Image	Note
TS25: brain	Weak			Very low expression in brain.

Structure	Level	Pattern	Image	Note
TS26: neural retina	Strong			Very abundant expression in embryonic retinoblastic cell layer. No expression in differentiated ganglion cell layer.
TS26: spinal cord ventricular layer	Present
TS26: spinal cord marginal layer	Absent
TS26: dorsal root ganglion	Absent
TS26: choroid plexus	Trace			Expression is barely above background.
TS26: bone	Absent			No expression in osteoblasts.

Structure	Level	Pattern	Image	Note
TS26: brain	Weak	Regionally restricted		Very low expression in brain.
TS26: brain ventricular layer	Weak
TS26: brain marginal layer	Absent			No expression in intermediate or marginal layers.
TS26: choroid plexus	Trace
TS26: thymus	Very strong	Regionally restricted		Expression localized mainly to cortical region which contains immature thymocytes.
TS26: metanephros	Present
TS26: liver	Strong
TS26: heart	Present
TS26: limb	Present
TS26: bone	Absent			No expression in osteoblasts.