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Gene Expression Data
Assay Details
Assay
Reference: J:138816 Plusa B, et al., Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst. Development. 2008 Sep;135(18):3081-91
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5776497
Gene symbol: Cdx2
Gene name: caudal type homeobox 2
Antibody: anti-Cdx2 (CDX2-88)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen S3A: embryonic day 3.5 (more )
Note: 33-cell early blastocyst; double labeled: red - Cdx2; white - Nanog (assay MGI:5776486).
Structure Level Pattern Image Note
TS4: trophectoderm Present S3A merge , S3A Cdx2 Expressing cells may also express Nanog.
TS4: inner cell mass Absent S3A Cdx2 , S3A merge
Specimen S3B: embryonic day 3.5 (more )
Note: 32-cell early blastocyst; double labeled: red - Cdx2; white - Nanog (assay MGI:5776486).
Structure Level Pattern Image Note
TS4: trophectoderm Present S3B Cdx2 , S3B merge Expressing cells may also express Nanog.
TS4: inner cell mass Present Single cells S3B Cdx2 , S3B merge In rare cases expression is seen in inner cell mass cells.
Specimen S3C: embryonic day 4.0 (more )
Note: 60-cell expanded blastocyst; double labeled: red - Cdx2; white - Nanog (assay MGI:5776486).
Structure Level Pattern Image Note
TS5: trophectoderm Present S3C Cdx2 , S3C merge
TS5: inner cell mass Absent S3C merge , S3C Cdx2
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory