Probe:	aB-crystallin probe
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS28: decidua	Present
TS11: embryo	Absent
TS11: extraembryonic component	Absent

Structure	Level	Pattern	Image	Note
TS28: decidua	Present
TS12: embryo	Absent
TS12: extraembryonic component	Absent

Structure	Level	Pattern	Image	Note
TS13: cardiac muscle tissue	Present		2A/B	Expression is in cardiac myocytes.
TS13: heart	Present	Regionally restricted	2A/B	Expression is in cardiac myocytes.
TS13: endocardial tube	Absent		2A/B	There is no expression in the endocardium, as shown at high magnification in the inset.
TS13: amnion	Weak		2A/B
TS28: decidua	Present		2A/B
TS13: trophectoderm	Absent		2A/B	There is no expression in the trophoblastic epithelium.

Structure	Level	Pattern	Image	Note
TS15: heart atrium	Present	Uniform	2C/D
TS15: primitive ventricle	Present	Uniform	2C/D
TS28: decidua	Present		2C/D
TS15: chorioallantoic placenta	Absent		2C/D	There is no expression in the fetal placenta.

Structure	Level	Pattern	Image	Note
TS17: heart atrium	Present	Uniform	3A/B
TS17: primitive ventricle	Present	Uniform	3A/B
TS17: endocardial cushion	Absent		3A/B	No expression in the endocardial cushion cells in the atrioventricular canal.
TS17: trunk myotome	Present	Uniform	3A/B	Expression is in the dorso-ventral aspect.
TS17: trunk dermatome	Absent		3A/B

Structure	Level	Pattern	Image	Note
TS17: umbilical cord mesenchyme	Weak			There are low levels of expression in mesenchymal cells surrounding the umbilical vessels.
TS17: chorioallantoic placenta	Weak	Regionally restricted		There are low levels of expression in the allantoic mesenchyme.
TS17: labyrinthine zone	Absent			No expression in labyrinth trophoblast.
TS17: trophoblast giant cell	Absent

Structure	Level	Pattern	Image	Note
TS19: heart atrium	Present		3C/D
TS19: heart ventricle	Present		3C/D
TS19: myotome	Present		3C/D	Prominent expression is seen distally.
TS19: spinal nerve	Present		3C/D , 3E/F	Expression is seen as the nerves exit from the dorsal root ganglion and pass between the vertebral primordia. It may be in Schwann cells.
TS19: dorsal root ganglion	Absent		3C/D , 3E/F
TS19: trigeminal nerve	Present		3C/D	Expression is seen in the 5th cranial nerve.
TS19: trigeminal ganglion	Absent		3C/D	No expression seen in the Gasserian ganglion.
TS19: skeletal musculature	Present	Graded	3C/D	Expression is seen in developing axial musculature ventral to the developing vertebral column of the neck, thorax, abdomen and tail.

Structure	Level	Pattern	Image	Note
TS20: atrium cardiac muscle	Strong	Uniform	4A/B	Expression is seen in both the trabecular and compact layers.
TS20: ventricle cardiac muscle	Strong	Uniform	4A/B	Expression is seen in both the trabecular and compact layers.
TS20: endocardial cushion	Absent		4A/B
TS20: heart valve primordium	Absent		4A/B	No expression in the aortic valve.
TS20: outflow tract aortic component	Absent		4A/B	No expression in the aortic outflow.
TS20: skeletal musculature	Present		4A/B	Expression is seen in cervical muscles.

Structure	Level	Pattern	Image	Note
TS20: myotome	Present		4C/D
TS20: forelimb mesenchyme	Present		4C/D	Expression seen in forelimb proximal pre muscle masses.

Structure	Level	Pattern	Image	Note
TS20: lung	Absent
TS20: metanephros	Absent

Structure	Level	Pattern	Image	Note
TS21: heart atrium	Strong		5A/B
TS21: heart ventricle	Strong		5A/B
TS21: pharynx mesenchyme	Present		5A/B	Expression seen in pharynx muscles.
TS21: skeletal musculature	Present		5A/B	Expression seen in neck muscles.
TS21: tongue extrinsic pre-muscle mass	Present		5A/B
TS21: tongue intrinsic muscle	Present		5A/B
TS21: cranial musculature	Present		5A/B	Expression seen in the muscle bundle of the proximal mandible.

Structure	Level	Pattern	Image	Note
TS22: heart atrium	Present	Uniform	6A/B
TS22: heart ventricle	Present	Uniform	6A/B
TS22: pulmonary trunk	Absent		6A/B
TS22: pulmonary valve	Absent		6A/B
TS22: intercostal muscle	Strong		6A/B

Structure	Level	Pattern	Image	Note
TS23: heart atrium	Present		6C/D
TS23: heart ventricle	Present		6C/D
TS23: pulmonary vein	Present		6C/D	The veins contain extracardiac myocytes.
TS23: intercostal muscle	Strong		6C/D
TS23: diaphragm	Strong		6C/D

Structure	Level	Pattern	Image	Note
TS24: hindlimb muscle	Present