Probe:	Shh probe8
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS23: retina	Present	Regionally restricted	1_Shh E14.5	Expression confined to inner half of central retina.

Structure	Level	Pattern	Image	Note
TS25: retina inner nuclear layer	Present	Regionally restricted	1_Shh E17	Expression clearly localized to developing ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present		1_Shh P7	From P7 onwards, expression localized to both ganglion cell layer and inner nuclear layer.
TS28: retina inner nuclear layer	Present	Regionally restricted	1_Shh P7	Expression localized to a subset of cells in inner half of inner nuclear layer, possibly amacrine cells.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present		1_Shh P14	From P7 onwards, expression localized to both ganglion cell layer and inner nuclear layer.
TS28: retina inner nuclear layer	Present	Regionally restricted	1_Shh P14	Expression localized to a subset of cells in inner half of inner nuclear layer, possibly amacrine cells.

Structure	Level	Pattern	Image	Note
TS28: retina ganglion cell layer	Present			Authors state that the pattern of expression is maintained in the adult.
TS28: retina inner nuclear layer	Present	Regionally restricted		Authors state that the pattern of expression is maintained in the adult.