Probe:	2BX
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	It is possible that another probe (6EH, MGI:16095) has been used. Though the 2BX gave a somewhat better signal, no significant differences were observed between the results obtained with the two probes.

Structure	Level	Pattern	Image	Note
TS14: embryo mesenchyme	Present		1A/B

Structure	Level	Pattern	Image	Note
TS16: limb mesenchyme	Present	Regionally restricted	1D/E	Limited to undifferentiated mesenchyme, presumably in those cells that give rise to skeletal tissues.

Structure	Level	Pattern	Image	Note
TS18: tail mesenchyme	Present	Regionally restricted	1G/H	Limited to undifferentiated mesenchyme, presumably in those cells that give rise to skeletal tissues.

Structure	Level	Pattern	Image	Note
TS21: gut mesenchyme	Present		1J/K

Structure	Level	Pattern	Image	Note
TS23: lung mesenchyme	Present		1M/N

Structure	Level	Pattern	Image	Note
TS22: basioccipital cartilage condensation	Present	Regionally restricted	2A/B	Expressed in the occipital bone, in the inner layer of the perichondrium.

Structure	Level	Pattern	Image	Note
TS24: basioccipital bone	Present	Regionally restricted	2D/E	Expressed in the occipital bone, in the periosteum and at the site of osteogenesis in the zone of endochondral ossification.

Structure	Level	Pattern	Image	Note
TS21: vertebral cartilage condensation	Present		2G/H

Structure	Level	Pattern	Image	Note
TS24: femur	Moderate	Regionally restricted	3A/B	Expressed in the zone of endochondral ossification. Expressed by neither the proliferating nor the hypertrophic chondrocytes.

Structure	Level	Pattern	Image	Note
TS24: fibula	Moderate	Regionally restricted	3D/E	Expressed in the zone of endochondral ossification. Expressed by neither the proliferating nor the hypertrophic chondrocytes.
TS24: tibia	Moderate	Regionally restricted	3D/E	Expressed in the zone of endochondral ossification. Expressed by neither the proliferating nor the hypertrophic chondrocytes.

Structure	Level	Pattern	Image	Note
TS27: alveolar process	Moderate		3G/H

Structure	Level	Pattern	Image	Note
TS27: long bone	Present	Regionally restricted	4A	Expressed in osteogenic cells.

Structure	Level	Pattern	Image	Note
TS27: long bone	Present	Regionally restricted	4C	Expressed in hematopoietic cells.

Structure	Level	Pattern	Image	Note
TS14: future brain	Present	Regionally restricted	5A/B	Expressed in the ependymal cell layer.

Structure	Level	Pattern	Image	Note
TS18: brain	Present	Regionally restricted	5D/E	Expressed in the ependymal cell layer.

Structure	Level	Pattern	Image	Note
TS18: head mesenchyme	Strong	Regionally restricted	6A/B	Expressed in differentiating cells of the frontonasal mesenchyme.

Structure	Level	Pattern	Image	Note
TS24: vibrissa follicle	Present	Regionally restricted	6D/E	Expressed in the outer root sheath.

Structure	Level	Pattern	Image	Note
TS27: vibrissa follicle	Present	Regionally restricted	6G/H	Expressed in the outer root sheath.

Structure	Level	Pattern	Image	Note
TS12: embryo mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS16: organ system	Present	Regionally restricted		Expressed in the mesenchyme of the craniofacial region at the site of future whisker follicles.
TS16: 1st branchial arch mandibular component	Moderate	Regionally restricted		Expressed in the mandible.
TS16: head mesenchyme	Strong	Regionally restricted		Expressed in differentiating cells of the frontonasal mesenchyme and in the mesenchyme of the craniofacial region at the site of future whisker follicles.

Structure	Level	Pattern	Image	Note
TS18: future spinal cord	Present	Regionally restricted		Expressed in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS20: tail paraxial mesenchyme	Present	Regionally restricted		Limited to undifferentiated mesenchyme, presumably in those cells that give rise to skeletal tissues.

Structure	Level	Pattern	Image	Note
TS21: lung mesenchyme	Present
TS21: rib cartilage condensation	Present
TS21: basioccipital pre-cartilage condensation	Present			Expressed in the occipital bone.
TS21: femur cartilage condensation	Moderate	Regionally restricted		Expressed in the inner perichondrium. Expressed by neither the proliferating nor the hypertrophic chondrocytes.
TS21: humerus cartilage condensation	Moderate	Regionally restricted		Expressed in the inner perichondrium. Expressed by neither the proliferating nor the hypertrophic chondrocytes.
TS21: radius-ulna cartilage condensation	Moderate	Regionally restricted		Expressed in the inner perichondrium. Expressed by neither the proliferating nor the hypertrophic chondrocytes.
TS21: tibia-fibula pre-cartilage condensation	Moderate	Regionally restricted		Expressed in the inner perichondrium. Expressed by neither the proliferating nor the hypertrophic chondrocytes.
TS21: spinal cord	Present	Regionally restricted		Expressed in the mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: vibrissa follicle	Present	Regionally restricted		Expressed in the mesenchyme.
TS22: appendicular skeleton	Present
TS22: vertebral cartilage condensation	Present
TS22: cranium	Moderate	Regionally restricted		Expressed in the temporal bone region.
TS22: head mesenchyme	Present	Regionally restricted		Expressed in differentiating cells of the frontonasal mesenchyme.

Structure	Level	Pattern	Image	Note
TS23: ilium	Present	Regionally restricted		Expressed in cells of the inner perichondrium.
TS23: scapula	Present	Regionally restricted		Expressed in cells of the inner perichondrium.
TS23: cranium	Moderate	Regionally restricted		Expressed in the temporal bone region.
TS23: vibrissa follicle	Present	Regionally restricted		Expressed in the outer root sheath.

Structure	Level	Pattern	Image	Note
TS24: ilium	Present	Regionally restricted		Expressed in the periosteum and the zone of endochondral ossification.
TS24: scapula	Present	Regionally restricted		Expressed in the periosteum and the zone of endochondral ossification.
TS24: spinal cord	Present	Regionally restricted		Expressed in the mesenchyme.
TS24: brain	Present	Regionally restricted		Expressed in the ependymal cell layer.

Structure	Level	Pattern	Image	Note
TS25: embryo mesenchyme	Present
TS25: lung mesenchyme	Present

Structure	Level	Pattern	Image	Note
TS27: metanephros	Present	Regionally restricted		Expressed in the mesenchyme. Expression becomes less abundant as embryogenesis progresses.
TS27: clavicle	Present
TS27: mandible	Present
TS27: occipital bone	Present
TS27: rib	Present
TS27: sphenoid bone	Present
TS27: vertebra	Present
TS27: ilium	Present	Regionally restricted		Expressed in the periosteum and the zone of endochondral ossification. Expressed by neither the proliferating nor the hypertrophic chondrocytes.
TS27: scapula	Present	Regionally restricted		Expressed in the periosteum and the zone of endochondral ossification. Expressed by neither the proliferating nor the hypertrophic chondrocytes.
TS27: femur	Moderate	Regionally restricted		Expressed in the inner periosteum and the osteogenic diaphysis. Expressed by neither the proliferating nor the hypertrophic chondrocytes.
TS27: fibula	Moderate	Regionally restricted		Expressed in the inner periosteum and the osteogenic diaphysis. Expressed by neither the proliferating nor the hypertrophic chondrocytes.
TS27: humerus	Moderate	Regionally restricted		Expressed in the inner periosteum and the osteogenic diaphysis. Expressed by neither the proliferating nor the hypertrophic chondrocytes.
TS27: radius	Moderate	Regionally restricted		Expressed in the inner periosteum and the osteogenic diaphysis. Expressed by neither the proliferating nor the hypertrophic chondrocytes.
TS27: tibia	Moderate	Regionally restricted		Expressed in the inner periosteum and the osteogenic diaphysis. Expressed by neither the proliferating nor the hypertrophic chondrocytes.
TS27: ulna	Moderate	Regionally restricted		Expressed in the inner periosteum and the osteogenic diaphysis. Expressed by neither the proliferating nor the hypertrophic chondrocytes.