Gene Expression Data
Assay Details
Assay
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Reference:
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J:147050
Nishioka N, et al., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev Cell. 2009 Mar;16(3):398-410
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5763606
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Gene symbol:
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Wwtr1
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Gene name:
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WW domain containing transcription regulator 1
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Antibody:
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Anti-TAZ (2149)
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
3E 32:
embryonic day 3.5
(more )
Note: 32-cell. Double labeled: green - Wwtr1; red - Cdx2 (assay MGI:5763604).
Specimen
3E 32:
(close )
Genetic Background:
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involves: C57BL/6 * DBA
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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32-cell. Double labeled: green - Wwtr1; red - Cdx2 (assay MGI:5763604).
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Structure |
Level |
Pattern |
Image |
Note |
TS4: compacted morula |
Present |
Non-Uniform |
3E Wwtr1 32
,
3E merge 32
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High levels were detected in ouside cell nuclei; low, but detectable levels were detected in inside cell nuclei.
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Specimen
3E 40:
embryonic day 3.5
(more )
Note: 40-cell. Double labeled: green - Wwtr1; red - Cdx2 (assay MGI:5763604).
Specimen
3E 40:
(close )
Genetic Background:
|
involves: C57BL/6 * DBA
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Age:
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embryonic day 3.5
|
Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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40-cell. Double labeled: green - Wwtr1; red - Cdx2 (assay MGI:5763604).
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Structure |
Level |
Pattern |
Image |
Note |
TS4: blastocyst |
Present |
Non-Uniform |
3E merge 40
,
3E Wwtr1 40
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High levels were detected in ouside cell nuclei; low, but detectable levels were detected in inside cell nuclei.
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