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Gene Expression Data
Assay Details
Assay
Reference: J:56240 Lichanska AM, et al., Differentiation of the mononuclear phagocyte system during mouse embryogenesis: the role of transcription factor PU.1. Blood. 1999 Jul 1;94(1):127-38
Assay type: RNA in situ
MGI Accession ID: MGI:5762997
Gene symbol: Spi1
Gene name: Spi-1 proto-oncogene
Probe: PU.1 probe2
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 4A: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: liver Present 4A
TS19: embryo Present Spotted 4A Detected as a fine speckled pattern throughout the embryo.
TS19: body wall Present Regionally restricted 4A Expressed in cells flanking the neural tube at the bottom of the field.
Specimen 4B: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: body wall mesenchyme Present Regionally restricted 4B Expressed at intervals along the dorsal midline in mesenchyme surrounding the spinal cord.
Specimen 4C: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: embryo Present 4C Positive staining cells contains multiple foci staining indicating phagocytosis of pyknotic cells.
Specimen 4D: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: limb Present Regionally restricted 4D Expressed at the limb margins.
Specimen not shown: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: embryo Absent
TS15: yolk sac Absent
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory