Gene Expression Data
Assay Details
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Assay
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Reference:
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J:193320
Keller LV, et al., Restoring physiological cell heterogeneity in the mesenchyme during tooth engineering. Int J Dev Biol. 2012;56(9):737-46
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5762813
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Gene symbol:
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Ptprc
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Gene name:
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protein tyrosine phosphatase receptor type C
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Antibody:
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anti-CD45
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
2E:
embryonic day 14.5
(more  )
Note: Double labeled: green - CD45 (Ptprc); red - collagen IV.
Specimen
2E:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - CD45 (Ptprc); red - collagen IV.
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Specimen
4A:
embryonic day 14.5
(more )
Note: cap stage; double labeled: green - CD45 (Ptprc); red - CD31 (Pecam1 assay MGI:5762805).
Specimen
4A:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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cap stage; double labeled: green - CD45 (Ptprc); red - CD31 (Pecam1 assay MGI:5762805).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lower jaw molar mesenchyme |
Present |
Single cells |
4A
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Expression in very few cells at the periphery of the condensed mesenchyme. The pattern of expressing cells was clearly distinct from that of blood vessels.
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Specimen
4B:
embryonic day 18.5
(more )
Note: early bell stage; double labeled: green - CD45 (Ptprc); red - CD31 (Pecam1 assay MGI:5762805).
Specimen
4B:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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early bell stage; double labeled: green - CD45 (Ptprc); red - CD31 (Pecam1 assay MGI:5762805).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: lower jaw molar mesenchyme |
Present |
Regionally restricted |
4B
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Expression in an increased number of cells, mainly in the peridental mesenchyme. The pattern of expressing cells was clearly distinct from that of blood vessels.
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Specimen
4C:
postnatal day 1
(more )
Note: late bell stage; double labeled: green - CD45 (Ptprc); red - CD31 (Pecam1 assay MGI:5762805).
Specimen
4C:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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late bell stage; double labeled: green - CD45 (Ptprc); red - CD31 (Pecam1 assay MGI:5762805).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: lower jaw molar mesenchyme |
Present |
Regionally restricted |
4C
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The number of expressing cells increased from ED18 to PN4. The pattern of expressing cells was clearly distinct from that of blood vessels
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Specimen
4D:
postnatal day 4
(more )
Note: late bell stage; double labeled: green - CD45 (Ptprc); red - CD31 (Pecam1 assay MGI:5762805).
Specimen
4D:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 4
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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late bell stage; double labeled: green - CD45 (Ptprc); red - CD31 (Pecam1 assay MGI:5762805).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: lower jaw molar mesenchyme |
Present |
Regionally restricted |
4D
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The number of expressing cells increased from ED18 to PN4. The pattern of expressing cells was clearly distinct from that of blood vessels
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Specimen
4Q:
postnatal day 4
(more )
Note: late bell stage; double labeled: green - CD45 (Ptprc); red - CD31 (Pecam1 assay MGI:5762805).
Specimen
4Q:
(close )
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Genetic Background:
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CD-1
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Age:
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postnatal day 4
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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late bell stage; double labeled: green - CD45 (Ptprc); red - CD31 (Pecam1 assay MGI:5762805).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: lower jaw molar mesenchyme |
Present |
Regionally restricted |
4Q
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The number of expressing cells increased from ED18 to PN4. The pattern of expressing cells was clearly distinct from that of blood vessels
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Analysis Tools