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Gene Expression Data
Assay Details
Assay
Reference: J:194321 Nott A, et al., S-nitrosylation of HDAC2 regulates the expression of the chromatin-remodeling factor Brm during radial neuron migration. Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):3113-8
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5762594
Gene symbol: Nos1
Gene name: nitric oxide synthase 1, neuronal
Antibody: anti-NOS1 (A-11): sc-5302
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 1A: embryonic day 15.5 (more )
Note: Double labeled: green - Nos1; red - Hdac2 (assay MGI:5762608).
Structure Level Pattern Image Note
TS23: cerebral cortex intermediate zone Present 1A left , 1A right Expressed in the cytoplasm.
Specimen 4A: embryonic day 18.5 (more )
Note: Double labeled: green - Nos1; red - Smarca2 (assay MGI:5762610).
Structure Level Pattern Image Note
TS26: cerebral cortex intermediate zone Present 4A nNOS , 4A Merge
Specimen S1a E12.5: embryonic day 12.5 (more )
Note: Double labeled: green - Nos1; red - Hdac2 (assay MGI:5762608).
Structure Level Pattern Image Note
TS20: cerebral cortex Ambiguous S1a E12.5 nNOS , S1a E12.5 Merge Little or no staining was detected.
TS20: cerebral cortex ventricular layer Absent S1a E12.5 Merge , S1a E12.5 nNOS
Specimen S1a E15.5: embryonic day 15.5 (more )
Note: Double labeled: green - Nos1; red - Hdac2 (assay MGI:5762608).
Structure Level Pattern Image Note
TS23: cerebral cortex intermediate zone Present S1a E15.5 nNOS , S1a E15.5 Merge
TS23: cortical plate Present S1a E15.5 nNOS , S1a E15.5 Merge
TS23: cerebral cortex ventricular layer Absent S1a E15.5 nNOS , S1a E15.5 Merge
Specimen S1a E18.5: embryonic day 18.5 (more )
Note: Double labeled: green - Nos1; red - Hdac2 (assay MGI:5762608).
Structure Level Pattern Image Note
TS26: cerebral cortex intermediate zone Present S1a E18.5 Merge , S1a E18.5 nNOS
TS26: cortical plate Present S1a E18.5 Merge , S1a E18.5 nNOS
TS26: cerebral cortex ventricular zone Absent S1a E18.5 Merge , S1a E18.5 nNOS
Specimen S1b WT: embryonic day 18.5 (more )
Note: Double labeled: green - Nos1; red - Hdac2 (assay MGI:5762608).
Structure Level Pattern Image Note
TS26: cerebral cortex Present S1b left
Specimen S1b nNOS-/-: embryonic day 18.5; Nos1tm1Plh/Nos1tm1Plh (more )
Note: Double labeled: green - Nos1; red - Hdac2 (assay MGI:5762608).
Structure Level Pattern Image Note
TS26: cerebral cortex Absent S1b right
Specimen S8a E12: embryonic day 12.5 (more )
Note: Double labeled: green - Nos1; red - Smarca2 (assay MGI:5762610).
Structure Level Pattern Image Note
TS20: cerebral cortex Present S8a E12 merge , S8a E12 nNOS
Specimen S8a E15: embryonic day 15.5 (more )
Note: Double labeled: green - Nos1; red - Smarca2 (assay MGI:5762610).
Structure Level Pattern Image Note
TS23: cerebral cortex Present S8a E15 nNOS , S8a E15 merge
Specimen S8a E18: embryonic day 18.5 (more )
Note: Double labeled: green - Nos1; red - Smarca2 (assay MGI:5762610).
Structure Level Pattern Image Note
TS26: cerebral cortex Present S8a E18 merge , S8a E18 nNOS
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
04/23/2024
MGI 6.23 		The Jackson Laboratory