Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:39208
Sosa-Pineda B, et al., The Pax4 gene is essential for differentiation of insulin-producing beta cells in the mammalian pancreas. Nature. 1997 Mar 27;386(6623):399-402
|
Assay type:
|
In situ reporter (knock in)
|
MGI Accession ID:
|
MGI:5755864
|
Gene symbol:
|
Pax4
|
Gene name:
|
paired box 4
|
Reporter:
|
lacZ
|
|
Direct Detection
|
Assay notes:
|
Specimens were post-fixed with 4% paraformaldehyde.
|
|
Results
|
Specimen
2a:
embryonic day 10.5; Pax4tm1Pgr/Pax4 +
(more )
Specimen
2a:
(close )
Genetic Background:
|
either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * NMRI)
|
Mutant Allele(s):
|
Pax4tm1Pgr/Pax4+
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Specimen
2b:
postnatal day 0; Pax4tm1Pgr/Pax4 +
(more )
Note: Double labeled: blue - Pax4 reporter; brown - Ins1 (assay MGI:5756064) and Ins2 (assay MGI:5756065).
Specimen
2b:
(close )
Genetic Background:
|
either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * NMRI)
|
Mutant Allele(s):
|
Pax4tm1Pgr/Pax4+
|
Age:
|
postnatal day 0
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: blue - Pax4 reporter; brown - Ins1 (assay MGI:5756064) and Ins2 (assay MGI:5756065).
|
Specimen
2c:
postnatal day 0; Pax4tm1Pgr/Pax4 +
(more )
Note: Double labeled: blue - Pax4 reporter; brown - Gcg (assay MGI:5755996).
Specimen
2c:
(close )
Genetic Background:
|
either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * NMRI)
|
Mutant Allele(s):
|
Pax4tm1Pgr/Pax4+
|
Age:
|
postnatal day 0
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: blue - Pax4 reporter; brown - Gcg (assay MGI:5755996).
|
Specimen
2d:
(close )
Genetic Background:
|
either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * NMRI)
|
Mutant Allele(s):
|
Pax4tm1Pgr/Pax4tm1Pgr
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Specimen
2e:
(close )
Genetic Background:
|
either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * NMRI)
|
Mutant Allele(s):
|
Pax4tm1Pgr/Pax4tm1Pgr
|
Age:
|
postnatal day 0
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: blue - Pax4 reporter; brown - Ins1 (assay MGI:5756064) and Ins2 (assay MGI:5756065).
|
Specimen
2f:
(close )
Genetic Background:
|
either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * NMRI)
|
Mutant Allele(s):
|
Pax4tm1Pgr/Pax4tm1Pgr
|
Age:
|
postnatal day 0
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: blue - Pax4 reporter; brown - Gcg (assay MGI:5755996).
|
Specimen
not shown:
embryonic day 11.0; Pax4tm1Pgr/Pax4 +
(more )
Specimen
not shown:
(close )
Genetic Background:
|
either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * NMRI)
|
Mutant Allele(s):
|
Pax4tm1Pgr/Pax4+
|
Age:
|
embryonic day 11.0
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
not shown E16.5:
(close )
Genetic Background:
|
either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * NMRI)
|
Mutant Allele(s):
|
Pax4tm1Pgr/Pax4tm1Pgr
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
|