Gene Expression Data
Assay Details
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Assay
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Reference:
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J:41401
Grindley JC, et al., Disruption of PAX6 function in mice homozygous for the Pax6Sey-1Neu mutation produces abnormalities in the early development and regionalization of the diencephalon. Mech Dev. 1997 Jun;64(1-2):111-26
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5752909
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Gene symbol:
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Pax6
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Gene name:
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paired box 6
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Probe:
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Pax6 cDNA9
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Probe preparation:
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Antisense RNA
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Results
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Specimen
1A:
embryonic day 9.0
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future diencephalon |
Strong |
Regionally restricted |
1A
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Strong expression dorsally throughout presumptive diencephalon. Two regions of strong expression one just anterior to the forebrain-midbrain boundary, the other adjacent to and in the optic vesicles - with lower levels in the intervening region.
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Specimen
1B:
embryonic day 10.5
(more )
Specimen
1B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: diencephalon |
Present |
Regionally restricted |
1B
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Within field of expression in posterior diencephalon, close to forebrain-midbrain boundary, high levels of expression in bilaterally symmetric arrays of cells.
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Specimen
1D:
embryonic day 11.5
(more )
Specimen
1D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: diencephalon |
Present |
Regionally restricted |
1D
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Major changes in expression coincide with compartment boundaries.
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| TS19: prosomere 1 |
Strong |
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1D
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Strong expression in prosomere 1 (synencephalon)
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| TS19: prosomere 2 |
Present |
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1D
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Lower levels in prosomere 2 (presumptive dorsal thalamus). Strong expression in prospective epithalamus; lower levels laterally.
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| TS19: prosomere 3 |
Strong |
Regionally restricted |
1D
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Particularly strong expression prosomere 3 (presumptive ventral thalamus).
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| TS19: prethalamus |
Strong |
Regionally restricted |
1D
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Strongest expression in zona incerta, flanked ventrally and posteriorly by regions of lower expression.
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Specimen
3B:
embryonic day 11.5
(more )
Specimen
3B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6 control:
embryonic day 11.5
(more )
Specimen
6 control:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
6 Sey/Sey:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: diencephalon |
Present |
Regionally restricted |
6_Sey/Sey
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Anterior expression with diffuse posterior limit. Expression absent from posterior diencephalon.
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