Gene Expression Data
Assay Details
Assay
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Reference:
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J:35540
Bouillet P, et al., A new mouse member of the Wnt gene family, mWnt-8, is expressed during early embryogenesis and is ectopically induced by retinoic acid. Mech Dev. 1996 Aug;58(1-2):141-52
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5752299
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Gene symbol:
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Wnt8a
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Gene name:
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wingless-type MMTV integration site family, member 8A
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Probe:
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Stra11 cDNA1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
5A (mWnt-8):
embryonic day 6.75
(more )
Note: Early streak stage.
Specimen
5A (mWnt-8):
(close )
Genetic Background:
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involves: C57BL/6 * SJL
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Age:
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embryonic day 6.75
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Early streak stage.
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Specimen
5B:
embryonic day 7.25
(more )
Note: Late streak stage.
Specimen
5B:
(close )
Genetic Background:
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involves: C57BL/6 * SJL
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Age:
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embryonic day 7.25
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Late streak stage.
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Structure |
Level |
Pattern |
Image |
Note |
TS10: embryo ectoderm |
Present |
Regionally restricted |
5B
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Specific expression in a wide posterior region of embryonic ectoderm. Expression gradually decreased toward lateral regions of epiblast.
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TS10: primitive streak |
Present |
Regionally restricted |
5B
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Expression extends in proximal part of primitive streak.
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TS10: embryo mesoderm |
Absent |
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5B
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No clear labeling in mesoderm exiting from streak.
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Specimen
5C:
embryonic day 7.25
(more )
Note: Late streak stage.
Specimen
5C:
(close )
Genetic Background:
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involves: C57BL/6 * SJL
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Age:
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embryonic day 7.25
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Late streak stage.
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Structure |
Level |
Pattern |
Image |
Note |
TS10: embryo ectoderm |
Present |
Regionally restricted |
5C
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Specific expression in a wide posterior region of embryonic ectoderm. Expression gradually decreased toward lateral regions of epiblast.
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TS10: embryo mesoderm |
Absent |
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5C
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No clear labeling in mesoderm exiting from streak.
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Specimen
5D:
embryonic day 7.25
(more )
Note: Late streak stage.
Specimen
5D:
(close )
Genetic Background:
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involves: C57BL/6 * SJL
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Age:
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embryonic day 7.25
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Late streak stage.
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Specimen
5E:
embryonic day 8.0
(more )
Note: Late headfold stage.
Specimen
5E:
(close )
Genetic Background:
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involves: C57BL/6 * SJL
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Late headfold stage.
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Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo |
Present |
Regionally restricted |
5E
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Sharp rostral boundary of expression at base of developing headfolds.
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Specimen
5F:
embryonic day 8.0
(more )
Note: Late headfold stage.
Specimen
5F:
(close )
Genetic Background:
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involves: C57BL/6 * SJL
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Late headfold stage.
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Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo mesoderm |
Weak |
Regionally restricted |
5F
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Expression at lower levels in mesoderm of posterior regions.
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Specimen
5G:
embryonic day 8.0
(more )
Note: Late headfold stage.
Specimen
5G:
(close )
Genetic Background:
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involves: C57BL/6 * SJL
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Late headfold stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo mesoderm |
Weak |
Regionally restricted |
5G
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Expression at lower levels in mesoderm of posterior regions.
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Specimen
6B:
embryonic day 7.5
(more )
Specimen
6B:
(close )
Genetic Background:
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involves: C57BL/6 * SJL
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo ectoderm |
Present |
Regionally restricted |
6B
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Expression extended from distal tip of conceptus to posterior limit of embryo, and toward lateral and rostral regions of ectoderm.
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TS11: embryo mesoderm |
Weak |
Regionally restricted |
6B
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Expression at lower levels in mesoderm of posterior regions.
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TS11: extraembryonic component |
Absent |
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6B
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No expression in allantois or other extraembryonic regions.
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Specimen
6F:
embryonic day 7.75
(more )
Specimen
6F:
(close )
Genetic Background:
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involves: C57BL/6 * SJL
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Age:
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embryonic day 7.75
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Sex:
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Not Specified
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Type:
|
section
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS11: embryo mesoderm |
Present |
Regionally restricted |
6F
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Expression in primitive streak region, both in ectoderm and newly formed mesoderm.
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TS11: primitive streak |
Present |
Regionally restricted |
6F
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Expression in primitive streak region, both in ectoderm and newly formed mesoderm.
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TS11: embryo ectoderm |
Present |
Regionally restricted |
6F
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Expression extends rostrally within the ectoderm.
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TS11: embryo |
Present |
Regionally restricted |
6F
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Expression ends at the embryonic/extraembryonic border delimited by the attachment site of amniotic membrane.
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TS11: extraembryonic component |
Absent |
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6F
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No expression in allantois or other extraembryonic regions.
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