Lifr RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:34635 Nichols J, et al., Complementary tissue-specific expression of LIF and LIF-receptor mRNAs in early mouse embryogenesis. Mech Dev. 1996 Jul;57(2):123-31
Assay type:	RNA in situ
MGI Accession ID:	MGI:5752175
Gene symbol:	Lifr
Gene name:	LIF receptor alpha

Probe:	Lifr probe1
Probe preparation:	Antisense labelled with S35 RNA
Assay notes:	Probe was labeled with either S35 or P32.

Results

Specimen 2B: embryonic day 3.5 (more

)

Structure	Level	Pattern	Image	Note
TS4: inner cell mass	Present		2B
TS4: trophectoderm	Absent

Specimen 3B/E embryo: embryonic day 5.5 (more

)

Structure	Level	Pattern	Image	Note
TS7: embryo	Absent		3B , 3E

Specimen 3B/E decidua: postnatal adult (more

)
Note: Specimen from pregnant mouse at 5.5 dpc.

Structure	Level	Pattern	Image	Note
TS28: decidua	Strong	Regionally restricted	3B , 3E	Relatively high level of expression in deciduum, distributed in gradient with highest levels in regions immediately surrounding the embryo.

Specimen 4B/E: embryonic day 8.5 (more

)

Structure	Level	Pattern	Image	Note
TS13: amnion	Present		4E , 4B
TS13: neural tube	Present		4E , 4B
TS13: somite	Present		4E , 4B
TS13: trunk unsegmented mesenchyme	Present		4E , 4B
TS13: yolk sac	Present	Regionally restricted	4E , 4B	Expression in visceral yolk sac.

Specimen not shown E8.5 uterus: postnatal adult (more

)
Note: Specimen from pregnant mouse at 8.5 dpc.

Structure	Level	Pattern	Image	Note
TS28: decidua	Weak	Regionally restricted		Authors report expression in decidua decreases with time, becoming restricted to a small area near the placenta by 8.5 dpc.
TS28: uterine gland	Present

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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