Gene Expression Data
Assay Details
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Assay
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Reference:
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J:116230
Huber K, et al., Cholinergic differentiation occurs early in mouse sympathetic neurons and requires Phox2b. Gene Expr. 2006;13(2):133-9
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5751425
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Gene symbol:
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Chat
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Gene name:
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choline O-acetyltransferase
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Probe:
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ChAT probe
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1C:
embryonic day 11.5
(more  )
Specimen
1C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1F:
embryonic day 11.5
(more )
Specimen
1F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1I:
embryonic day 11.5
(more )
Specimen
1I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: sympathetic ganglion |
Present |
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1I
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Expression is in the lower thoracic to lumbal adrenal and sympathetic ganglion anlagen.
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Specimen
3F:
embryonic day 13.5
(more )
Specimen
3F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: adrenal gland |
Present |
Single cells |
3F
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Expression is detected in only a few cells of adrenal tissue.
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| TS21: sympathetic ganglion |
Present |
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3F
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Expression is in ganglion cell clusters dorsal and ventral to the adrenal anlagen.
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Specimen
4B:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Phox2btm1Jbr/Phox2btm1Jbr
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
4E:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Phox2btm1Jbr/Phox2btm1Jbr
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: sympathetic ganglion |
Absent |
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4E
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Shown is the lower thoracic to lumbal adrenal and sympathetic ganglion anlagen.
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Specimen
not shown:
embryonic day 10.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
not shown +/-:
embryonic day 11.5; Phox2btm1Jbr/Phox2b +
(more )
Specimen
not shown +/-:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Phox2btm1Jbr/Phox2b+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools