Probe:	Ihh cDNA6
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: radius-ulna pre-cartilage condensation	Present	Regionally restricted	9A
TS20: forelimb digit 2	Present	Regionally restricted	9A	Authors state there is one segment of expression in digits 2-4.
TS20: forelimb digit 3	Present	Regionally restricted	9A	Authors state there is one segment of expression in digits 2-4.
TS20: forelimb digit 4	Present	Regionally restricted	9A	Authors state there is one segment of expression in digits 2-4.

Structure	Level	Pattern	Image	Note
TS20: radius-ulna pre-cartilage condensation	Present	Regionally restricted	9B
TS20: forelimb digit 2	Present	Regionally restricted	9B	Authors state there is one segment of expression in digits 2-4.
TS20: forelimb digit 3	Present	Regionally restricted	9B	Authors state there is one segment of expression in digits 2-4.
TS20: forelimb digit 4	Present	Regionally restricted	9B	Authors state there is one segment of expression in digits 2-4.

Structure	Level	Pattern	Image	Note
TS23: forelimb digit 2	Present	Regionally restricted	9C	Four segments in expression in digits 2-5.
TS23: forelimb digit 3	Present	Regionally restricted	9C	Four segments in expression in digits 2-5.
TS23: forelimb digit 4	Present	Regionally restricted	9C	Four segments in expression in digits 2-5.
TS23: forelimb digit 5	Present	Regionally restricted	9C	Four segments in expression in digits 2-5.
TS23: radius	Present	Regionally restricted	9C	Expression split into two clearly separated ends in the ulna and radius.
TS23: ulna	Present	Regionally restricted	9C	Expression split into two clearly separated ends in the ulna and radius.

Structure	Level	Pattern	Image	Note
TS23: forelimb digit 2	Present	Regionally restricted	9D	Authors report only three segments of expression in digits.
TS23: forelimb digit 3	Present	Regionally restricted	9D	Authors report only three segments of expression in digits.
TS23: forelimb digit 4	Present	Regionally restricted	9D	Authors report only three segments of expression in digits.
TS23: forelimb digit 5	Present	Regionally restricted	9D	Authors report only three segments of expression in digits.
TS23: radius	Present	Regionally restricted	9D	Spacing of expression is almost absent in the mutant. Expression in ulna and radius closely spaced and near the middle of forming bones.
TS23: ulna	Present	Regionally restricted	9D	Spacing of expression is almost absent in the mutant. Expression in ulna and radius closely spaced and near the middle of forming bones.