Rho Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:161846 Westfall JE, et al., Retinal degeneration and failure of photoreceptor outer segment formation in mice with targeted deletion of the Joubert syndrome gene, Ahi1. J Neurosci. 2010 Jun 30;30(26):8759-68
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5751130
Gene symbol:	Rho
Gene name:	rhodopsin

Antibody:	4D2
Detection system:	Secondary antibody
Assay notes:	Fixative was 1% or 4 % paraformaldehyde.

Results

3A Ahi1+/+ ©

Specimen 3A Ahi1+/+: embryonic day 18.5 (more

)

Structure	Level	Pattern	Image	Note
TS26: neural retina	Present	Regionally restricted	3A Ahi1+/+	Expression is in some cells of the photoreceptor layer.

3A Ahi1-/- ©

Specimen 3A Ahi1-/-: embryonic day 18.5; Ahi1^tm1Rujf/Ahi1^tm1Rujf (more

)

Structure	Level	Pattern	Image	Note
TS26: neural retina	Present		3A Ahi1-/-	Expression in this mutant is the same as in wild-type.

5A Ahi1+/+ PN11 ©

Specimen 5A +/+ PN11: postnatal day 11 (more

)

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer outer segment	Strong		5A Ahi1+/+ PN11

5A Ahi1+/+ PN22 ©

Specimen 5A +/+ PN22: postnatal day 22 (more

)

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer outer segment	Strong		5A Ahi1+/+ PN22

5A Ahi1+/+ PN42 ©

Specimen 5A +/+ PN42: postnatal day 42 (more

)

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer outer segment	Strong		5A Ahi1+/+ PN42

5A Ahi1-/- PN11 ©

Specimen 5A -/- PN11: postnatal day 11; Ahi1^tm1Rujf/Ahi1^tm1Rujf (more

)

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer outer segment	Strong	Regionally restricted	5A Ahi1-/- PN11	Expression was absent in the outermost layers of the outer nuclear layer.
TS28: photoreceptor layer inner segment	Strong		5A Ahi1-/- PN11

5A Ahi1-/- PN22 ©

Specimen 5A -/- PN22: postnatal day 22; Ahi1^tm1Rujf/Ahi1^tm1Rujf (more

)

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer outer segment	Strong	Regionally restricted	5A Ahi1-/- PN22	Expression was absent in the outermost layers of the outer nuclear layer.
TS28: photoreceptor layer inner segment	Strong		5A Ahi1-/- PN22

5A Ahi1-/- PN42 ©

Specimen 5A -/- PN42: postnatal day 42; Ahi1^tm1Rujf/Ahi1^tm1Rujf (more

)

Structure	Level	Pattern	Image	Note
TS28: photoreceptor layer outer segment	Strong	Single cells	5A Ahi1-/- PN42	Expression was absent in the outermost layers of the outer nuclear layer.
TS28: photoreceptor layer inner segment	Strong		5A Ahi1-/- PN42

5B Ahi1+/+ ©

Specimen 5B Ahi1+/+: postnatal day 11 (more

)

Structure	Level	Pattern	Image	Note
TS28: retina	Present		5B Ahi1+/+

5B Ahi1-/- ©

Specimen 5B Ahi1-/-: postnatal day 11; Ahi1^tm1Rujf/Ahi1^tm1Rujf (more

)

Structure	Level	Pattern	Image	Note
TS28: retina	Present		5B Ahi1-/-

6A ©

Specimen 6A: postnatal (more

)

Structure	Level	Pattern	Image	Note
TS28: retina	Present		6A

6B ©

Specimen 6B: postnatal; Ahi1^tm1Rujf/Ahi1^tm1Rujf (more

)

Structure	Level	Pattern	Image	Note
TS28: retina	Present		6B

Specimen 8A Ahi1+/+: embryonic day 18.5 (more

)
Note: Double labeled: red - Rho; green - Rab8a (assay MGI:5751265).

Structure	Level	Pattern	Image	Note
TS26: retina	Present		8A Ahi1+/+ rhodopsin , 8A Ahi1+/+ merge

Specimen 8A Ahi1-/-: embryonic day 18.5; Ahi1^tm1Rujf/Ahi1^tm1Rujf (more

)
Note: Double labeled: red - Rho; green - Rab8a (assay MGI:5751265).

Structure	Level	Pattern	Image	Note
TS26: retina	Present		8A Ahi1-/- merge , 8A Ahi1-/- rhodopsin

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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