Probe:	Spag5 probe2
Probe preparation:	labelled with digoxigenin

Structure	Level	Pattern	Image	Note
TS20: forebrain ventricular layer	Present		3B
TS20: liver	Present		3B
TS20: lung	Present		3B
TS20: metanephros	Present		3B

Structure	Level	Pattern	Image	Note
TS20: cochlea	Present
TS20: heart	Present
TS20: intestine	Present
TS20: limb	Present
TS20: midbrain	Present
TS20: olfactory epithelium	Present
TS20: testis	Present
TS20: vibrissa follicle	Present
TS20: gland	Present	Regionally restricted		Expression in adrenal gland, thymus, and submandibular gland.
TS20: jaw primordium	Present			Expression in upper and lower jaw.

Structure	Level	Pattern	Image	Note
TS22: adrenal gland	Present		3C
TS22: forebrain ventricular layer	Present		3C
TS22: limb	Present		3C
TS22: liver	Present		3C
TS22: lung	Present		3C
TS22: metanephros	Present		3C
TS22: midbrain	Present		3C
TS22: gland	Present	Regionally restricted	3C	Expression in thymus and submandibular gland.

Structure	Level	Pattern	Image	Note
TS22: heart	Present
TS22: intestine	Present
TS22: lower jaw	Present
TS22: olfactory epithelium	Present
TS22: testis	Present
TS22: upper jaw	Present
TS22: vibrissa follicle	Present

Structure	Level	Pattern	Image	Note
TS24: eye	Present		3D
TS24: forebrain ventricular layer	Present		3D
TS24: liver	Present		3D
TS24: metanephros	Present		3D
TS24: midbrain	Present		3D

Structure	Level	Pattern	Image	Note
TS24: lung	Present
TS24: thymus	Present
TS24: vibrissa follicle	Present

Structure	Level	Pattern	Image	Note
TS22: embryo	Present		3E

Structure	Level	Pattern	Image	Note
TS20: neural retinal epithelium	Strong	Regionally restricted	3F	Strong expression in the eye from E12.5-18.5 in the neuroblastic layer of the retinStrong expression in the eye from E12.5-18.5 in the neuroblastic layer of the retina.

Structure	Level	Pattern	Image	Note
TS22: neural retinal epithelium	Strong	Regionally restricted	3G	Strong expression in the eye from E12.5-18.5 in the neuroblastic layer of the retina.

Structure	Level	Pattern	Image	Note
TS24: retina nuclear layer	Strong		3H	Strong expression in the eye from E12.5-18.5 in the neuroblastic layer of the retinaStrong expression in the eye from E12.5-18.5 in the neuroblastic layer of the retina.

Structure	Level	Pattern	Image	Note
TS24: retina inner nuclear layer	Present		3I
TS24: neural retina	Present	Regionally restricted	3I	Much weaker expression in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS26: retina nuclear layer	Strong		3J	Strong expression in the neuroblastic layer of the retina.

Structure	Level	Pattern	Image	Note
TS26: retina inner nuclear layer	Present		3K
TS26: neural retina	Present	Regionally restricted	3K	Much weaker expression in the ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS28: neural retina	Present	Regionally restricted	3L	Expression maintained at postnatal day 7.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present		3M
TS28: retina ganglion cell layer	Weak		3M	Much weaker expression in the ganglion cell layer.