Antibody:	anti-Pitx1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS28: adenohypophysis pars anterior	Present	Non-Uniform	4A	All cells in the anterior pituitary express at either high or low levels. Eosin-stained acidophils (somatotrophs and lactotrophs) have weak immunostaining.

Structure	Level	Pattern	Image	Note
TS28: adenohypophysis pars intermedia	Weak

Structure	Level	Pattern	Image	Note
TS28: adenohypophysis pars anterior	Present		4D	There was good correlation of highly-expressing cells with Cga expression (gonadotrophs and thyrotrophs).

Structure	Level	Pattern	Image	Note
TS28: adenohypophysis pars anterior	Present		4E	About 15% of the intensely labeled cells also express Tshb.

Structure	Level	Pattern	Image	Note
TS28: adenohypophysis pars anterior	Present		4F	A proportion of intensely immunopositive cells co-expressed Lhb.

Structure	Level	Pattern	Image	Note
TS28: adenohypophysis pars anterior	Present		4G	Most of the highly expressing cells did not co-express Pomc.

Structure	Level	Pattern	Image	Note
TS20: pituitary gland	Present	Graded	4H	A graded signal was detected from rostral to caudal.
TS20: adenohypophysis pars intermedia	Trace		4H
TS20: adenohypophysis pars tuberalis	Strong		4H

Structure	Level	Pattern	Image	Note
TS24: adenohypophysis pars tuberalis	Strong		4I
TS24: adenohypophysis pars anterior	Present	Scattered	4I

Structure	Level	Pattern	Image	Note
TS24: pituitary gland	Present	Regionally restricted	4J	Highly expressing cells co-express Cga. Most intensely labeled nuclei are found in the ventral part of the gland.

Structure	Level	Pattern	Image	Note
TS24: adenohypophysis pars tuberalis	Strong	Non-Uniform	4K	There were intensely labeled cells that did not co-express Pomc. However, corticotrophs expressing high levels were mostly found in the ventral region of the gland.

Structure	Level	Pattern	Image	Note
TS24: pituitary gland	Present	Non-Uniform	4L	There were intensely- and weakly-labeled cells that co-expressed Pomc. Corticotrophs expressing high levels were mostly found in the ventral region of the gland.