Probe:	Ptx1 cDNA3
Probe preparation:	Antisense RNA
Assay notes:	Two probes were described that gave identical results MGI:2150936 and MGI:2150937; either may have been used in this assay.

Structure	Level	Pattern	Image	Note
TS17: Rathke's pouch	Present	Uniform	1A/A'

Structure	Level	Pattern	Image	Note
TS24: adenohypophysis pars tuberalis	Strong		1B/B'
TS24: adenohypophysis pars anterior	Present	Scattered	1B/B'	Scattered cells in the ventral region of the anterior lobe exhibited higher expression levels.

Structure	Level	Pattern	Image	Note
TS23: adenohypophysis pars tuberalis	Strong		1C/C'
TS23: head mesenchyme	Absent		1C/C'	There was no expression in the angiogenic mesenchyme that will form the capillary bed of the gland at the junction between the pars tuberalis and pars distalis.

Structure	Level	Pattern	Image	Note
TS21: adenohypophysis pars tuberalis	Present		2A/A'

Structure	Level	Pattern	Image	Note
TS19: Rathke's pouch	Present	Non-Uniform	2B/B'	Expression was down-regulated from the Rathke's pouch on E11.5-13.5 in the anterior lobe but not in the pars tuberalis.