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Gene Expression Data
Assay Details
Assay
Reference: J:216290 Yeung J, et al., Wls provides a new compartmental view of the rhombic lip in mouse cerebellar development. J Neurosci. 2014 Sep 10;34(37):12527-37
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5707892
Gene symbol: Atoh1
Gene name: atonal bHLH transcription factor 1
Reporter: lacZ
Antibody: anti-B-galactosidase
Results
Specimen 4A: embryonic day 11.5; Atoh1tm2Hzo/Atoh1+ (more )
Note: Double labeled: green - Atoh1; red - Wls (assay MGI:5707888).
Structure Level Pattern Image Note
TS19: cerebellum primordium Present Regionally restricted 4A center , 4A right Expression was detected at the surface of the cerebellar anlage. There was no overlapping of expression with Wls.

Specimen 4B: embryonic day 11.5; Atoh1tm2Hzo/Atoh1+ (more )
Note: Double labeled: green - Atoh1; red - Lmx1a (assay MGI:5707891).
Structure Level Pattern Image Note
TS19: cerebellum primordium Present Regionally restricted 4B right , 4B center Expression was detected at the surface of the cerebellar anlage. A minimal number of cells showed coexpression with Lmx1a.

Specimen 5B/C: embryonic day 13.5; Atoh1tm2Hzo/Atoh1+ (more )
Note: Double labeled: green - Atoh1; red - Wls (assay MGI:5707888).
Structure Level Pattern Image Note
TS21: cerebellum Strong Regionally restricted 5B , 5C Expression was strong in the emerging external granule cell layer.
TS21: rhombic lip Present Regionally restricted 5B , 5C Columns of expression were detected in the rhombic lip. There was some overlap with Wls expression.

Specimen 5E/F: embryonic day 15.5; Atoh1tm2Hzo/Atoh1+ (more )
Note: Double labeled: green - Atoh1; red - Wls (assay MGI:5707888).
Structure Level Pattern Image Note
TS23: cerebellum external granule cell layer Present 5F , 5E
TS23: rhombic lip Present 5F , 5E Expression was detected in the exterior face of the rhombic lip, continuous with the forming external granule cell layer. Expression was largely non-overlapping with Wls with only a few co-expressing cells.

Specimen 5H/I: embryonic day 18.5; Atoh1tm2Hzo/Atoh1+ (more )
Note: Double labeled: green - Atoh1; red - Wls (assay MGI:5707888).
Structure Level Pattern Image Note
TS26: cerebellum external granule cell layer Present 5I , 5H There was no co-expression with Wls.

Specimen 6: embryonic day 15.5; Atoh1tm2Hzo/Atoh1tm2Hzo (more )
Note: Double labeled: green - Atoh1; red - Wls (assay MGI:5707888).
Structure Level Pattern Image Note
TS23: rhombic lip Present Regionally restricted 6_center , 6_right LacZ reporter was detected in the rhombic lip. Co-expression with Wls was seen in the interior face of the rhombic lip in many more cells in the mutant compared to the wild type.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory