Wls Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:216290 Yeung J, et al., Wls provides a new compartmental view of the rhombic lip in mouse cerebellar development. J Neurosci. 2014 Sep 10;34(37):12527-37
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:5707885
Gene symbol:	Wls
Gene name:	wntless WNT ligand secretion mediator

Antibody:	Anti-Gpr177 (WLAP-177)
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Two antibodies were described. The N-terminus antibody MGI:5707881 and C-terminus antibody MGI:5707874. The expression patterns were identical between the two antibodies.

Results

1D ©

Specimen 1D: embryonic day 13.5 (more

)

Structure	Level	Image
TS21: 4th ventricle choroid plexus	Present	1D
TS21: hindbrain meninges	Present	1D
TS21: hindbrain roof	Present	1D
TS21: lower rhombic lip	Present	1D
TS21: midbrain-hindbrain junction	Present	1D
TS21: upper rhombic lip	Present	1D

1E ©

Specimen 1E: embryonic day 15.5 (more

)

Structure	Level	Image
TS23: 4th ventricle choroid plexus	Present	1E
TS23: hindbrain meninges	Present	1E
TS23: hindbrain roof	Present	1E
TS23: lower rhombic lip	Present	1E
TS23: midbrain-hindbrain junction	Present	1E
TS23: upper rhombic lip	Present	1E

1F ©

Specimen 1F: embryonic day 18.5 (more

)

Structure	Level	Image
TS26: 4th ventricle choroid plexus	Present	1F
TS26: hindbrain meninges	Present	1F
TS26: hindbrain roof	Present	1F
TS26: lower rhombic lip	Present	1F
TS26: midbrain-hindbrain junction	Present	1F
TS26: upper rhombic lip	Present	1F

Specimen not shown: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Weak

Specimen 8 +/+ E13.5: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: rhombic lip	Present		8_E13.5 +/+ immuno

Specimen 8 Sey/Sey E13.5: embryonic day 13.5; Pax6^Sey/Pax6^Sey (more

)

Structure	Level	Pattern	Image	Note
TS21: rhombic lip	Present		8_E13.5 Sey/Sey immuno	Expression was detected as in wild type.

Specimen 8 +/+ E15.5: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: rhombic lip	Present		8_E15.5 +/+ immuno

Specimen 8 Sey/Sey E15.5: embryonic day 15.5; Pax6^Sey/Pax6^Sey (more

)

Structure	Level	Pattern	Image	Note
TS23: rhombic lip	Present		8_E15.5 Sey/Sey immuno
TS23: cerebellum external granule cell layer	Present		8_E15.5 Sey/Sey immuno	Expression in the mutant expanded into the external granule cell layer.

Specimen 8 +/+ E18.5: embryonic day 18.5 (more

)

Structure	Level	Pattern	Image	Note
TS26: rhombic lip	Weak		8_E18.5 +/+ immuno

Specimen 8 Sey/Sey E18.5: embryonic day 18.5; Pax6^Sey/Pax6^Sey (more

)

Structure	Level	Pattern	Image	Note
TS26: rhombic lip	Present		8_E18.5 Sey/Sey immuno
TS26: cerebellum external granule cell layer	Present		8_E18.5 Sey/Sey immuno	Expression in the mutant expanded into the external granule cell layer.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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