Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:139734
Hafler BP, et al., Expression and function of Nkx6.3 in vertebrate hindbrain. Brain Res. 2008 Jul 30;1222:42-50
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:5707712
|
Gene symbol:
|
Vsx2
|
Gene name:
|
visual system homeobox 2
|
Antibody:
|
Chx10 antibody
|
Detection system:
|
Secondary antibody coupled to Cy3
|
|
Results
|
Specimen
3E:
embryonic day 14.5; Nkx6-3tm1Shiv/Nkx6-3 +
(more )
Note: Double labeled: red - Vsx2; green - Gata3 (assay MGI:5707666).
Specimen
3E:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac
|
Mutant Allele(s):
|
Nkx6-3tm1Shiv/Nkx6-3+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Vsx2; green - Gata3 (assay MGI:5707666).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: hindbrain |
Present |
Regionally restricted |
3E
|
Expression is in V2a interneurons in the caudal hindbrain.
|
Specimen
3F:
embryonic day 14.5; Nkx6-3tm1Shiv/Nkx6-3 +
(more )
Note: Double labeled: red - Vsx2; green - Gata3 (assay MGI:5707666).
Specimen
3F:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac
|
Mutant Allele(s):
|
Nkx6-3tm1Shiv/Nkx6-3+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Vsx2; green - Gata3 (assay MGI:5707666).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: hindbrain |
Present |
Regionally restricted |
3F
|
Expression is in V2a interneurons in the caudal hindbrain.
|
Specimen
3G:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac
|
Mutant Allele(s):
|
Nkx6-3tm1Shiv/Nkx6-3tm1Shiv
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Vsx2; green - Gata3 (assay MGI:5707666).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: hindbrain |
Present |
Regionally restricted |
3G
|
Expression in this mutant is the same as in wild-type embryos.
|
Specimen
3H:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac
|
Mutant Allele(s):
|
Nkx6-3tm1Shiv/Nkx6-3tm1Shiv
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Vsx2; green - Gata3 (assay MGI:5707666).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: hindbrain |
Present |
Regionally restricted |
3H
|
Expression in this mutant is the same as in wild-type embryos.
|
Specimen
3I:
embryonic day 14.5; Nkx6-3tm1Shiv/Nkx6-3 +
(more )
Note: Double labeled: red - Vsx2; green - Mnx1 (assay MGI:5707668).
Specimen
3I:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac
|
Mutant Allele(s):
|
Nkx6-3tm1Shiv/Nkx6-3+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Vsx2; green - Mnx1 (assay MGI:5707668).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: hindbrain |
Present |
Regionally restricted |
3I
|
Expression is in V2a interneurons in the caudal hindbrain.
|
Specimen
3J:
embryonic day 14.5; Nkx6-3tm1Shiv/Nkx6-3 +
(more )
Note: Double labeled: red - Vsx2; green - Mnx1 (assay MGI:5707668).
Specimen
3J:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac
|
Mutant Allele(s):
|
Nkx6-3tm1Shiv/Nkx6-3+
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Vsx2; green - Mnx1 (assay MGI:5707668).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: hindbrain |
Present |
Regionally restricted |
3J
|
Expression is in V2a interneurons in the caudal hindbrain.
|
Specimen
3K:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac
|
Mutant Allele(s):
|
Nkx6-3tm1Shiv/Nkx6-3tm1Shiv
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Vsx2; green - Mnx1 (assay MGI:5707668).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: hindbrain |
Present |
Regionally restricted |
3K
|
Expression in this mutant is the same as in wild-type embryos.
|
Specimen
3L:
(close )
Genetic Background:
|
involves: 129S6/SvEvTac
|
Mutant Allele(s):
|
Nkx6-3tm1Shiv/Nkx6-3tm1Shiv
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
optical section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: red - Vsx2; green - Mnx1 (assay MGI:5707668).
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: hindbrain |
Present |
Regionally restricted |
3L
|
Expression in this mutant is the same as in wild-type embryos.
|
|