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Gene Expression Data
Assay Details
Assay
Reference: J:210446 Tekko T, et al., Initiation and developmental dynamics of Wfs1 expression in the context of neural differentiation and ER stress in mouse forebrain. Int J Dev Neurosci. 2014 Jun;35:80-8
Assay type: RT-PCR
MGI Accession ID: MGI:5704460
Gene symbol: Ddit3
Gene name: DNA-damage inducible transcript 3
Probe: CHOP_for, CHOP_rev
Probe preparation: labelled with SYBR green
Assay notes: Quantitative real-time PCR analysis. Relative expression to Hprt1 is shown as a table in the supplementary material.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
wt E17 E17.5 TS25: forebrain Present Not Specified; total RNA C57BL/6 Not Specified
wt P0 P0 TS27: forebrain Present (a) Not Specified; total RNA C57BL/6 Not Specified
wt P2 P2 TS27: forebrain Present Not Specified; total RNA C57BL/6 Not Specified
wt P4 P4 TS28: forebrain Present (b) Not Specified; total RNA C57BL/6 Not Specified
ko E17 E17.5 TS25: forebrain Present (c) Not Specified; total RNA involves: 129S6/SvEvTac Wfs1tm1Koks/Wfs1tm1KoksNot Specified
ko P0 P0 TS27: forebrain Present (c) Not Specified; total RNA involves: 129S6/SvEvTac Wfs1tm1Koks/Wfs1tm1KoksNot Specified
ko P2 P2 TS27: forebrain Present (c) Not Specified; total RNA involves: 129S6/SvEvTac Wfs1tm1Koks/Wfs1tm1KoksNot Specified
ko P4 P4 TS28: forebrain Present (c) Not Specified; total RNA involves: 129S6/SvEvTac Wfs1tm1Koks/Wfs1tm1KoksNot Specified
Notes:
(a) There was a 35% decrease in expression compared to E17.5.
(b) There was a 12% decrease in expression compared to P0.
(c) There were no statistical differences in expression between wild-type and mutant brains.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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