Procr In situ reporter (knock in) Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:217483 Wang D, et al., Identification of multipotent mammary stem cells by protein C receptor expression. Nature. 2015 Jan 1;517(7532):81-4
Assay type:	In situ reporter (knock in)
MGI Accession ID:	MGI:5703745
Gene symbol:	Procr
Gene name:	protein C receptor, endothelial

Reporter:	RFP
	Direct Detection

Results

Specimen 3b upper: postnatal; Procr^{tm1.1(cre/ERT2)Yaz}/Procr^{tm1.1(cre/ERT2)Yaz} (more

)
Note: Double labeled: red - Procr; green - Krt14 (assay MGI:5759971).

Structure	Level	Pattern	Image	Note
TS28: mammary gland epithelium	Present	Single cells	3b upper , 3b upper insert	Knock-in reporter expression is detected in rare cells in the basal layer.

Specimen 3b lower: postnatal; Procr^{tm1.1(cre/ERT2)Yaz}/Procr^{tm1.1(cre/ERT2)Yaz} (more

)
Note: Double labeled: red - Procr; green - Krt5 (assay MGI:5759972).

Structure	Level	Pattern	Image	Note
TS28: mammary gland epithelium	Present	Single cells	3b lower , 3b lower insert	Knock-in reporter expression is detected in rare cells in the basal layer.

Specimen Extended Data 4a: embryonic day 18.5; Procr^{tm1.1(cre/ERT2)Yaz}/Procr^{tm1.1(cre/ERT2)Yaz} (more

)
Note: Double labeled: red - Procr; green - Krt14 (assay MGI:5759971).

Structure	Level	Pattern	Image	Note
TS26: mammary gland epithelium	Present	Single cells	Extended Data 4a , Extended Data 4a2 , Extended Data 4a3 , Extended Data 4a1	Knock-in reporter expression is detected in the middle or at the tip of the mammary duct.

Specimen Extended Data 4b: postnatal day 1.5; Procr^{tm1.1(cre/ERT2)Yaz}/Procr^{tm1.1(cre/ERT2)Yaz} (more

)
Note: Double labeled: red - Procr; green - Krt14 (assay MGI:5759971).

Structure	Level	Pattern	Image	Note
TS27: mammary gland epithelium	Present	Single cells	Extended Data 4b , Extended Data 4b2 , Extended Data 4b1 , Extended Data 4b3	Knock-in reporter expression is detected in the middle or at the tip of the mammary duct.

Specimen Extended Data 4c: postnatal week 5; Procr^{tm1.1(cre/ERT2)Yaz}/Procr^{tm1.1(cre/ERT2)Yaz} (more

)
Note: terminal end bud; double labeled: red - Procr; green - Krt14 (assay MGI:5759971).

Structure	Level	Pattern	Image	Note
TS28: mammary gland duct	Absent		Extended Data 4c	Knock-in reporter expression is not detected in terminal end buds.

Specimen Extended Data 4d: postnatal week 5; Procr^{tm1.1(cre/ERT2)Yaz}/Procr^{tm1.1(cre/ERT2)Yaz} (more

)
Note: duct; double labeled: red - Procr; green - Krt14 (assay MGI:5759971).

Structure	Level	Pattern	Image	Note
TS28: mammary gland duct	Present	Single cells	Extended Data 4d1 , Extended Data 4d , Extended Data 4d2	Knock-in reporter expression is predominantly detected in the mammary ducts.

Specimen Extended Data 4e: postnatal week 8; Procr^{tm1.1(cre/ERT2)Yaz}/Procr^{tm1.1(cre/ERT2)Yaz} (more

)
Note: Double labeled: red - Procr; green - Krt14 (assay MGI:5759971).

Structure	Level	Pattern	Image	Note
TS28: mammary gland duct	Present		Extended Data 4e1 , Extended Data 4e2 , Extended Data 4e , Extended Data 4e3	Knock-in reporter expression is detected in cells located over the ducts.

Specimen Extended Data 4f: postnatal week 5; Procr^{tm1.1(cre/ERT2)Yaz}/Procr^{tm1.1(cre/ERT2)Yaz} (more

)
Note: terminal end bud; double labeled: red - Procr; blue - Krt5 (assay MGI:5759972). Dividing cells are labeled green (EdU).

Structure	Level	Pattern	Image	Note
TS28: mammary gland duct	Absent		Extended Data 4f	Knock-in reporter expression is not detected in terminal end buds.

Specimen Extended Data 4g: postnatal week 5; Procr^{tm1.1(cre/ERT2)Yaz}/Procr^{tm1.1(cre/ERT2)Yaz} (more

)
Note: duct; double labeled: red - Procr; blue - Krt5 (assay MGI:5759972). Dividing cells are labeled green (EdU).

Structure	Level	Pattern	Image	Note
TS28: mammary gland duct	Present	Single cells	Extended Data 4g	Knock-in reporter expression is detected in proliferating cells.

Specimen Extended Data 4h: postnatal week 8; Procr^{tm1.1(cre/ERT2)Yaz}/Procr^{tm1.1(cre/ERT2)Yaz} (more

)
Note: Double labeled: red - Procr; blue - Krt5 (assay MGI:5759972). Dividing cells are labeled green (EdU).

Structure	Level	Pattern	Image	Note
TS28: mammary gland	Present	Single cells	Extended Data 4h	Knock-in reporter expression is detected in proliferating cells.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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