Antibody:	anti-Cux1
Detection system:	Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Present	Regionally restricted	1C	Expression restricted to upper layers. Expression in the majority of cells of layers II-IV, but only in a few cells of the deep layers.
TS28: cortical layer II	Present	Regionally restricted	1C	Expression in neurons with pyramidally shaped cell bodies.
TS28: cortical layer III	Present	Regionally restricted	1C	Expression in neurons with pyramidally shaped cell bodies.
TS28: cortical layer IV	Present	Regionally restricted	1C	Expression in neurons with pyramidally shaped cell bodies.
TS28: cerebral cortex marginal zone	Absent		1C	Authors state that all cells of the marginal zone are negative for staining.

Structure	Level	Pattern	Image	Note
TS28: telencephalon	Present	Regionally restricted	1C inset	Authors report nuclear localization of protein.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present	Regionally restricted	2A , 2C	Expression localizes to the nucleus of most neurons.`
TS28: cortical layer III	Present	Regionally restricted	2A , 2C	Expression localizes to the nucleus of most neurons.`
TS28: cortical layer IV	Present	Regionally restricted	2A , 2C	Expression localizes to the nucleus of most neurons.`
TS28: cerebral cortex marginal zone	Absent		2C , 2A	Authors state that all cells of the marginal zone are negative for staining.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	2D	Expression in layers II-IV in neurons with pyramidally shaped cell bodies. No expression in some subpopulations of upper cortical neurons.

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present	Regionally restricted	2E , 2G	Expression in majority of neurons.
TS28: cortical layer III	Present	Regionally restricted	2E , 2G	Expression in majority of neurons.
TS28: cortical layer IV	Present	Regionally restricted	2E , 2G	Expression in majority of neurons.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	2H	Authors report no expression in parvalbumin-expressing cells.`

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present	Regionally restricted	3A	Expression in upper layers of cortex.
TS28: cortical layer III	Present	Regionally restricted	3A	Expression in upper layers of cortex.
TS28: cortical layer IV	Present	Regionally restricted	3A	Expression in upper layers of cortex.

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present	Regionally restricted	3C	Expression in late-born neurons located in the deeper cortex and do not form strict laminar boundaries.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex subventricular zone	Weak		4A	Authors report dim expression in ventricular zone and subventricular zone of developing telencephalon.
TS21: cerebral cortex ventricular layer	Weak		4A	Authors report dim expression in ventricular zone and subventricular zone of developing telencephalon.
TS21: cortical plate	Present	Single cells	4A	Expression only in a few cells below the marginal zone in the upper most part of the cortical plate. No expression in cells destined to form the deep cortical layers.
TS21: meninges	Present	Regionally restricted	4A	Authors report expression in cells of the pia, outside of the marginal zone.

Structure	Level	Pattern	Image	Note
TS21: cortical plate	Present	Regionally restricted	4A inset	Expression only in a few cells below the marginal zone in the upper most part of the cortical plate.`

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex subventricular zone	Weak		4B	Authors report dim expression in ventricular zone and subventricular zone of developing telencephalon.
TS23: cerebral cortex ventricular layer	Weak		4B	Authors report dim expression in ventricular zone and subventricular zone of developing telencephalon.
TS23: cerebral cortex intermediate zone	Present	Regionally restricted	4B	Expression in presumptive neurons migrating through the intermediate zone towards the cortical plate.
TS23: cortical plate	Present	Regionally restricted	4B

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	Present	Diffuse	5B	Diffuse expression in ventricular zone cell precursors.
TS23: cerebral cortex subventricular zone	Strong		5B	Intense expression in observed in the nucleus.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	Present	Regionally restricted	5C (top) VZ	Expression only slightly higher in the nucleus than outside the nucleus. Weak staining in cytoplasm, not evenly distributed.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex subventricular zone	Strong	Regionally restricted	5C (middle) SVZ	Intense expression in observed in the nucleus.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex subventricular zone	Present		5C (bottom)

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Present		8A

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex subventricular zone	Present		8B

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex subventricular zone	Present		8C

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex subventricular zone	Present		8D