Gene Expression Data
Assay Details
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Assay
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Reference:
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J:108240
Kusakabe T, et al., Origin of the ultimobranchial body cyst: T/ebp/Nkx2.1 expression is required for development and fusion of the ultimobranchial body to the thyroid. Dev Dyn. 2006 May;235(5):1300-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5700195
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Gene symbol:
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Calca
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Gene name:
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calcitonin/calcitonin-related polypeptide, alpha
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Antibody:
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anti-calcitonin
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Results
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Specimen
6A/C:
embryonic day 19.0
(more  )
Specimen
6A/C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 19.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: thyroid gland |
Present |
Scattered |
6C
,
6A
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Eventually distributed throughout the thyroid lobes.
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Specimen
6B/D:
embryonic day 19.0; Nkx2-1tm1Shk/Nkx2-1 +
(more )
Specimen
6B/D:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Nkx2-1tm1Shk/Nkx2-1+
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Age:
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embryonic day 19.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: thyroid gland |
Present |
Regionally restricted |
6D
,
6B
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Expressed in the ultimobranchial body cells, which appeared to be incompletely incorporated into the thyroid diverticulum and remained at the dorsal part of the thyroid lobe.
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Specimen
6E:
postnatal month 10; Nkx2-1tm1Shk/Nkx2-1 +
(more )
Specimen
6E:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Nkx2-1tm1Shk/Nkx2-1+
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Age:
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postnatal month 10
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: thyroid gland |
Strong |
Regionally restricted |
6E
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Expressed in some cells lining the vesicles and strong expression found in clumps of cells localized in the vicinity of the vesicles. No expression in the thyroid lobe.
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Specimen
7B:
embryonic day 19.0; Nkx2-1tm1Shk/Nkx2-1 +
(more )
Specimen
7B:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Nkx2-1tm1Shk/Nkx2-1+
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Age:
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embryonic day 19.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: thyroid gland |
Present |
Clusters |
7B
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Expressed in clusters of C-cell precursors.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Nkx2-1tm1Shk/Nkx2-1tm1Shk
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Age:
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embryonic day 19.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Analysis Tools