Antibody:	anti-Isl1 (K-20): sc-23590
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: spinal cord lateral wall	Present	Regionally restricted	2A	Expressed in the lateral part of the medial motor column. Only weak immunoreactivity was observed in the medial part of the medial motor column and in the lateral motor column.

Structure	Level	Pattern	Image	Note
TS21: spinal cord lateral wall	Strong	Regionally restricted	3A	Expressed strongly in the dorsolateral motoneurons, and moderately expressed much more in the medial part than in the lateral part at the rostral lumbar levels.

Structure	Level	Pattern	Image	Note
TS21: spinal cord lateral wall	Strong	Regionally restricted	3C	Strongly and moderately expressed in the dorsolateral and retrodorsolateral motoneurons at the L5-6 level. Those strongly immunoreactive were much fewer in the retrodorsolateral than in the dorsolateral nucleus.

Structure	Level	Pattern	Image	Note
TS21: spinal cord lateral wall	Present	Regionally restricted	3E	Expressed in both the sympathetic preganglionic neurons and the parasympathetic preganglionic neurons within the intermediolateral column.

Structure	Level	Pattern	Image	Note
TS22: spinal cord lateral wall	Strong	Regionally restricted	4A	Expressed strongly in the dorsolateral motoneurons, moderately expressed much more in the medial part than in the lateral part at the rostral lumbar levels.

Structure	Level	Pattern	Image	Note
TS22: spinal cord lateral wall	Strong	Regionally restricted	4C	Strongly and moderately expressed in the dorsolateral and retrodorsolateral motoneurons at the L5-6 level. Those strongly immunoreactive were much fewer in the retrodorsolateral than in the dorsolateral nucleus.

Structure	Level	Pattern	Image	Note
TS22: spinal cord lateral wall	Present	Regionally restricted	4E	Expressed in both the sympathetic preganglionic neurons and the parasympathetic preganglionic neurons within the intermediolateral column.

Structure	Level	Pattern	Image	Note
TS23: ventral grey horn	Present	Regionally restricted	5A	Only strongly expressed in a few motoneurons in the medial part of the dorsolateral nucleus at the rostral level.

Structure	Level	Pattern	Image	Note
TS23: ventral grey horn	Present	Regionally restricted	5C	Strongly and moderately expressed in the dorsolateral and retrodorsolateral motoneurons at the L5-6 level. Those strongly immunoreactive were much fewer in the retrodorsolateral and ventral nucleus than in the dorsolateral nucleus.

Structure	Level	Pattern	Image	Note
TS23: ventral grey horn	Present	Regionally restricted	5E	Expressed in the motoneurons of the caudal-most lumbar. More than moderate immunoreactivity was not detected in the parasympathetic preganglionic neurons of the intermediolateral column at the sacral levels.

Structure	Level	Pattern	Image	Note
TS23: ventral grey horn	Strong	Regionally restricted		Expressed in the sympathetic preganglionic neurons of the intermediolateral column.

Structure	Level	Pattern	Image	Note
TS24: ventral grey horn	Present			Degraded expression.