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Gene Expression Data
Assay Details
Assay
Reference: J:146871 Sadakata H, et al., SAP-1 is a microvillus-specific protein tyrosine phosphatase that modulates intestinal tumorigenesis. Genes Cells. 2009 Mar;14(3):295-308
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5698999
Gene symbol: Ptprh
Gene name: protein tyrosine phosphatase receptor type H
Antibody: SAP-1 (clone 123)
Detection system: Secondary antibody
Results
Specimen 2A: postnatal week 6 (more )
Note: Double labeled: red - Ptprh; green - Ctnnb1 (assay MGI:5699001).
Structure Level Pattern Image Note
TS28: jejunum epithelium Present 2A SAP-1 top , 2A Merge top , 2A Merge bottom , 2A SAP-1 bottom Expression is at the apical surface.
Specimen 2B: postnatal week 6 (more )
Structure Level Pattern Image Note
TS28: jejunum epithelium Present 2B SAP-1 Expression is at the apical surface.
Specimen 2C top: postnatal (more )
Note: Double labeled: green - Ptprh; red - Nherf1 (assay MGI:5699010).
Structure Level Pattern Image Note
TS28: jejunum epithelium Present 2C SAP-1 top , 2C Merge top
Specimen 2C bottom: postnatal (more )
Note: Double labeled with rhodamine-conjugated phalloidin (red).
Structure Level Pattern Image Note
TS28: jejunum epithelium Present 2C Merge bottom , 2C SAP-1 bottom
Specimen 2D WT: postnatal (more )
Note: Immuno-Electron Microscopy. Specimens were fixed in 4% paraformaldehyde and 1% glutaraldehyde, thick sections were immunolabeled with gold and post-fixed with 1% OsO4 and 0.1% ferrocyanide and embedded in Epon. Ulthrathin sections were examined by the electron microscope.
Structure Level Pattern Image Note
TS28: jejunum epithelium Present 2D WT Expression is at the microvilli.
Specimen 2D KO: postnatal; Ptprhtm1.1Mato/Ptprhtm1.1Mato (more )
Note: Immuno-Electron Microscopy. Specimens were fixed in 4% paraformaldehyde and 1% glutaraldehyde, thick sections were immunolabeled with gold and post-fixed with 1% OsO4 and 0.1% ferrocyanide and embedded in Epon. Ulthrathin sections were examined by the electron microscope.
Structure Level Pattern Image Note
TS28: jejunum epithelium Absent 2D KO
Specimen 3A Jejunum: postnatal (more )
Note: Double labeled with phalloidin (green).
Structure Level Pattern Image Note
TS28: jejunum epithelium Present 3A SAP-1 Jejunum , 3A Merge Jejunum Expression is at the apical surface.
Specimen 3A Stomach: postnatal week 6 (more )
Note: Double labeled with phalloidin (green).
Structure Level Pattern Image Note
TS28: stomach epithelium Weak 3A Merge Stomach , 3A SAP-1 Stomach Expression is at the apical surface of epithelial cells.
Specimen 3B: postnatal week 6 (more )
Note: Double labeled with Ulex europaeus agglutinin (green).
Structure Level Pattern Image Note
TS28: ileum epithelium Present 3B SAP-1 , 3B Merge Expression is at the microvilli and is absent in goblet cells.
Specimen 3D E14.5: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: intestine epithelium Absent 3D SAP-1 E14.5
Specimen 3D E16.5: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: intestine epithelium Weak 3D SAP-1 E16.5
Specimen 3D P1: postnatal day 1 (more )
Structure Level Pattern Image Note
TS27: intestine epithelium Present 3D SAP-1 P1
Specimen 3D P8: postnatal day 8 (more )
Structure Level Pattern Image Note
TS28: intestine epithelium Present 3D SAP-1 P8
Specimen 4A: postnatal week 6 (more )
Note: Double labeled: red - Ptprh; green - Ctnnb1 (assay MGI:5699001).
Structure Level Pattern Image Note
TS28: jejunum epithelium Present 4A b-catenin , 4A SAP-1
Specimen 4Ba,b: postnatal week 6 (more )
Note: Double labeled: red - Ptprh; green - Ctnnb1 (assay MGI:5699001).
Structure Level Pattern Image Note
TS28: jejunum epithelium Present Regionally restricted 4Bb , 4Ba Expression is at the villus tip and is absent at the crypts.
Specimen 4Bc,d: postnatal week 6 (more )
Note: Double labeled: red - Ptprh; green - Ctnnb1 (assay MGI:5699001).
Structure Level Pattern Image Note
TS28: jejunum epithelium Present Regionally restricted 4Bc , 4Bd Expression is at the villus tip and is absent at the crypts.
Specimen 5D WT: postnatal (more )
Note: Double labeled: red - Ptprh; green - Ctnnb1 (assay MGI:5699001).
Structure Level Pattern Image Note
TS28: jejunum epithelium Present 5D WT Expression is at the apical brush border.
Specimen 5D KO: postnatal; Ptprhtm1.1Mato/Ptprhtm1.1Mato (more )
Note: Double labeled: red - Ptprh; green - Ctnnb1 (assay MGI:5699001).
Structure Level Pattern Image Note
TS28: jejunum epithelium Absent 5D KO
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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